{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:22:02Z","timestamp":1785435722291,"version":"3.56.0"},"reference-count":59,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T00:00:00Z","timestamp":1763510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"The Key Research and Development Project of Henan Province","award":["251111113400"],"award-info":[{"award-number":["251111113400"]}]},{"name":"The key Research and Development Project of Henan Province","award":["252102110106"],"award-info":[{"award-number":["252102110106"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31701542"],"award-info":[{"award-number":["31701542"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2025,11,19]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Obesity has emerged as a significant global health threat. In this study, we investigated the anti-obesity properties of\n                      <jats:italic toggle=\"yes\">Clostridium butyricum<\/jats:italic>\n                      B-3, a strain noted for its high butyric acid production. This strain was administered to mice on a high-fat diet (HFD) to assess its potential anti-obesity effects. Our findings indicate that three different dosages of\n                      <jats:italic toggle=\"yes\">C. butyricum<\/jats:italic>\n                      B-3 significantly (\n                      <jats:italic toggle=\"yes\">P<\/jats:italic>\n                      &lt; 0.05) mitigated excessive weight gain and ameliorated kidney and liver damage in HFD mice. A medium dose (10\n                      <jats:sup>7<\/jats:sup>\n                      CFU\/day) of\n                      <jats:italic toggle=\"yes\">C. butyricum<\/jats:italic>\n                      B-3 markedly reduced levels of total cholesterol, triglycerides and low-density lipoprotein cholesterol, and elevated levels of high-density lipoprotein cholesterol in HFD mice. Additionally, this dosage lowered serum levels of inflammatory cytokines, including IL-6, IL-10, and TNF-\u03b1. Furthermore, treatment with\n                      <jats:italic toggle=\"yes\">C. butyricum<\/jats:italic>\n                      B-3 enhanced the diversity and relative abundance of gut microbiota in HFD-fed mice. Notably, the gut microbiota composition of the high-dose (10\n                      <jats:sup>8<\/jats:sup>\n                      CFU\/day) group closely resembled that of mice fed a normal diet.\n                      <jats:italic toggle=\"yes\">C. butyricum<\/jats:italic>\n                      B-3 also significantly increased the concentrations of acetic acid, propionic acid, and butyric acid in the gut. In summary,\n                      <jats:italic toggle=\"yes\">C. butyricum<\/jats:italic>\n                      B-3 demonstrated significant anti-obesity effects and the ability to regulate intestinal microbiota in HFD mice.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Obesity represents one of the most severe global health challenges of our time.\n                        <jats:italic toggle=\"yes\">Clostridium butyricum<\/jats:italic>\n                        has recently been recognized as a next-generation probiotic with considerable potential for applications in intestinal health, metabolic diseases, and neuroprotection. Our research focused on a specific strain of\n                        <jats:italic toggle=\"yes\">C. butyricum<\/jats:italic>\n                        known for its anti-obesity effects, which displayed robust capabilities in mitigating obesity and regulating intestinal flora. This strain holds significant promise for development in anti-obesity nutritional products and pharmaceuticals.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01152-25","type":"journal-article","created":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T14:04:10Z","timestamp":1760623450000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["The anti-obesity effects of\n                    <i>Clostridium butyricum<\/i>\n                    B-3 and its impact on gut microbiota"],"prefix":"10.1128","volume":"91","author":[{"given":"Yue","family":"Meng","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05sbgwt55","id-type":"ROR","asserted-by":"publisher"}],"name":"Food Engineering Technology Research Center\/Key Laboratory of Henan Province, Henan University of Technology","place":["Zhengzhou, 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