{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T03:07:53Z","timestamp":1790737673550,"version":"4.1.0"},"reference-count":56,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2019,10,15]],"date-time":"2019-10-15T00:00:00Z","timestamp":1571097600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2019,10,15]]},"abstract":"<jats:p>\n                    The yeast\n                    <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n                    is a commonly used microbial host for production of various biochemical compounds. From a physiological perspective, biosynthesis of these compounds competes with biomass formation in terms of carbon and\/or energy equivalents. Fermentation processes functioning at extremely low or near-zero growth rates would prevent loss of feedstock to biomass production. Establishing\n                    <jats:named-content content-type=\"genus-species\">S. cerevisiae<\/jats:named-content>\n                    cultures in which growth is restricted by the limited supply of a non-energy substrate therefore could have a wide range of industrial applications but remains largely unexplored. In this work we accomplished near-zero growth of\n                    <jats:named-content content-type=\"genus-species\">S. cerevisiae<\/jats:named-content>\n                    through limited supply of a non-energy nutrient, namely, the nitrogen or phosphorus source, and carried out a quantitative physiological study of the cells under these conditions. The possibility to achieve near-zero-growth\n                    <jats:named-content content-type=\"genus-species\">S. cerevisiae<\/jats:named-content>\n                    cultures through limited supply of a non-energy nutrient may offer interesting prospects to develop novel fermentation processes for high-yield production of bio-based chemicals.\n                  <\/jats:p>","DOI":"10.1128\/aem.01161-19","type":"journal-article","created":{"date-parts":[[2019,8,5]],"date-time":"2019-08-05T08:32:32Z","timestamp":1564993952000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Quantitative Physiology of Non-Energy-Limited Retentostat Cultures of Saccharomyces cerevisiae at Near-Zero Specific Growth Rates"],"prefix":"10.1128","volume":"85","author":[{"given":"Yaya","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Delft University of Technology, Delft, The 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