{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:49:42Z","timestamp":1791118182146,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2013,9,1]],"date-time":"2013-09-01T00:00:00Z","timestamp":1377993600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A stock of 148\n            <jats:named-content content-type=\"genus-species\">Cryptosporidium parvum<\/jats:named-content>\n            DNA extracts from lambs and goat kids selected from a previous study examining the occurrence of\n            <jats:named-content content-type=\"genus-species\">Cryptosporidium<\/jats:named-content>\n            species and GP60 subtypes in diarrheic lambs and goat kids in northeastern Spain was further characterized by a multilocus fragment typing approach with six mini- and microsatellite loci. Various degrees of polymorphism were seen at all but the MS5 locus, although all markers exhibited two major alleles accounting for more than 75% of isolates. A total of 56 multilocus subtypes (MLTs) from lambs (48 MLTs) and goat kids (11 MLTs) were identified. Individual isolates with mixed MLTs were detected on more than 25% of the farms, but most MLTs (33) were distinctive for individual farms, revealing the endemicity of cryptosporidial infections on sheep and goat farms. Comparison with a previous study in calves in northern Spain using the same six-locus subtyping scheme showed the presence of host-associated alleles, differences in the identity of major alleles, and very little overlap in MLTs between\n            <jats:named-content content-type=\"genus-species\">C. parvum<\/jats:named-content>\n            isolates from lambs and those from calves (1 MLT) or isolates from lambs and those from goat kids (3 MLTs). The Hunter-Gaston index of the multilocus technique was 0.976 (95% confidence interval [CI], 0.970 to 0.982), which supports its high discriminatory power for strain typing and epidemiological tracking. Population analyses revealed the presence of two host-associated subpopulations showing epidemic clonality among the\n            <jats:named-content content-type=\"genus-species\">C. parvum<\/jats:named-content>\n            isolates infecting calves and lambs\/goat kids, respectively, although evidence of genetic flow between the two subpopulations was also detected.\n          <\/jats:p>","DOI":"10.1128\/aem.01168-13","type":"journal-article","created":{"date-parts":[[2013,6,29]],"date-time":"2013-06-29T07:38:20Z","timestamp":1372491500000},"page":"5363-5371","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Host Association of Cryptosporidium parvum Populations Infecting Domestic Ruminants in Spain"],"prefix":"10.1128","volume":"79","author":[{"given":"Joaqu\u00edn","family":"Qu\u00edlez","sequence":"first","affiliation":[{"name":"Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"Vergara-Castiblanco","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luis","family":"Monteagudo","sequence":"additional","affiliation":[{"name":"Department of Anatomy, Embryology and Genetics, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilio","family":"del Cacho","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caridad","family":"S\u00e1nchez-Acedo","sequence":"additional","affiliation":[{"name":"Department of Animal Pathology, Faculty of Veterinary Sciences, University of Zaragoza, Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biotechadv.2008.02.003"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2009.03.018"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2005.06.008"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.2008.00377.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268809002295"},{"key":"e_1_3_2_7_2","first-page":"451","volume-title":"Cryptosporidium and cryptosporidiosis","author":"Sant\u00edn M","year":"2008","unstructured":"Sant\u00ednMTroutJM. 2008. 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