{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T01:04:54Z","timestamp":1792371894757,"version":"4.4.0"},"reference-count":74,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/doi.org\/10.1128\/ASMCopyrightv2"},{"start":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T00:00:00Z","timestamp":1698710400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Science, Technology and Innovation Commission of Shenzhen Municipality","award":["JCYJ20200109142416497"],"award-info":[{"award-number":["JCYJ20200109142416497"]}]},{"name":"MOST | National Key Research and Development Program of China","award":["2021YFA0717003"],"award-info":[{"award-number":["2021YFA0717003"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2023,10,31]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Outer membrane vesicle (OMV)-delivered\n            <jats:italic>Pseudomonas<\/jats:italic>\n            quinolone signal (PQS) plays a critical role in cell\u2013cell communication in\n            <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n            . However, the functions and mechanisms of membrane-enclosed PQS in interspecies communication in microbial communities are not clear. Here, we demonstrate that PQS delivered by both OMVs from\n            <jats:italic>P. aeruginosa<\/jats:italic>\n            and liposome reduces the competitiveness of\n            <jats:italic>Burkholderia cenocepacia<\/jats:italic>\n            , which usually shares the same niche in the lungs of cystic fibrosis patients, by interfering with quorum sensing (QS) in\n            <jats:italic>B. cenocepacia<\/jats:italic>\n            through the LysR-type regulator ShvR. Intriguingly, we found that ShvR regulates the production of the QS signals cis-2-dodecenoic acid (BDSF) and N-acyl homoserine lactone (AHL) by directly binding to the promoters of signal synthase-encoding genes. Perception of PQS influences the regulatory activity of ShvR and thus ultimately reduces QS signal production and virulence in\n            <jats:italic>B. cenocepacia<\/jats:italic>\n            . Our findings provide insights into the interspecies communication mediated by the membrane-enclosed QS signal among bacterial species residing in the same microbial community.\n          <\/jats:p>\n          <jats:sec>\n            <jats:title>IMPORTANCE<\/jats:title>\n            <jats:p>\n              Quorum sensing (QS) is a ubiquitous cell-to-cell communication mechanism. Previous studies showed that\n              <jats:italic>Burkholderia cenocepacia<\/jats:italic>\n              mainly employs cis-2-dodecenoic acid (BDSF) and N-acyl homoserine lactone (AHL) QS systems to regulate biological functions and virulence. Here, we demonstrate that\n              <jats:italic>Pseudomonas<\/jats:italic>\n              quinolone signal (PQS) delivered by outer membrane vesicles from\n              <jats:italic>Pseudomonas aeruginosa<\/jats:italic>\n              or liposome attenuates\n              <jats:italic>B. cenocepacia<\/jats:italic>\n              virulence by targeting the LysR-type regulator ShvR, which regulates the production of the QS signals BDSF and AHL in\n              <jats:italic>B. cenocepaci<\/jats:italic>\n              a. Our results not only suggest the important roles of membrane-enclosed PQS in interspecies and interkingdom communications but also provide a new perspective on the use of functional nanocarriers loaded with QS inhibitors for treating pathogen infections.\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1128\/aem.01184-23","type":"journal-article","created":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T13:00:16Z","timestamp":1696510816000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":12,"title":["Membrane-enclosed\n            <i>Pseudomonas<\/i>\n            quinolone signal attenuates bacterial virulence by interfering with quorum sensing"],"prefix":"10.1128","volume":"89","author":[{"given":"Xia","family":"Li","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Sciences (Shenzhen), Shenzhen Campus of Sun Yat-sen University, Sun Yat-sen University, Shenzhen, Guangdong, 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