{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,12]],"date-time":"2026-07-12T02:08:48Z","timestamp":1783822128796,"version":"3.55.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The mechanism of whole-genome duplication (WGD) in yeast has been intensively studied because it has a large impact on yeast evolution. WGD has shaped the genomic architecture of modern\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n            ; however, the mechanism for restoring fertility after interspecies hybridization, which would be involved in the process of WGD, has not been thoroughly elucidated. In this study, we obtained a draft genome sequence of the salt-tolerant yeast\n            <jats:named-content content-type=\"genus-species\">Zygosaccharomyces rouxii<\/jats:named-content>\n            NBRC110957 and revealed that it is a hybrid lineage of\n            <jats:named-content content-type=\"genus-species\">Z. rouxii<\/jats:named-content>\n            (allodiploid) with two subgenomes equivalent to NBRC1876. Because this allodiploid yeast can mate with other allodiploid strains and form spores, it can be a good model of restoring fertility after interspecies hybridization. We observed that NBRC110957 and NBRC1876 contain six mating-type-like (\n            <jats:italic>MTL<\/jats:italic>\n            ) loci. There are no large deletions or deleterious mutations in\n            <jats:italic>MTL<\/jats:italic>\n            loci, except for several-base-pair deletions in the X region in certain\n            <jats:italic>MTL<\/jats:italic>\n            loci. We also assigned only one mating-type (MAT) locus that exclusively determines mating types from six\n            <jats:italic>MTL<\/jats:italic>\n            loci. These results suggest that it is possible to recover mating competence regardless of whether cells lose one\n            <jats:italic>MAT<\/jats:italic>\n            locus through random gene loss by mitotically dividing after interspecies hybridization. Moreover, we propose that perturbation of gene expression and substantial breakdown of\n            <jats:italic>MAT<\/jats:italic>\n            heterozygosity caused by chromosomal rearrangement at\n            <jats:italic>MTL<\/jats:italic>\n            loci play roles in restoring the mating competence of allodiploids. This scenario can provide a mechanism for restoring fertility after interspecies hybridization that is compatible with random gene loss models and suggests genomic plasticity during WGD in yeast.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            A whole-genome duplication occurred in an ancestor of the baker's yeast\n            <jats:named-content content-type=\"genus-species\">Saccharomyces cerevisiae<\/jats:named-content>\n            . The origins of this complex and multifaceted process, which requires intra- or interspecies hybridization followed by dysfunction of one mating-type (\n            <jats:italic>MAT<\/jats:italic>\n            ) locus to regain mating competence, has not been thoroughly elucidated. In this study, we provide a mechanism for regaining fertility in an interspecies hybrid,\n            <jats:named-content content-type=\"genus-species\">Zygosaccharomyces rouxii<\/jats:named-content>\n            . The draft genome sequence analysis and mating test showed that the\n            <jats:named-content content-type=\"genus-species\">Z. rouxii<\/jats:named-content>\n            strain used in this study is an intact interspecies hybrid, suggesting that it is possible to recover fertility regardless of whether cells lose one\n            <jats:italic>MAT<\/jats:italic>\n            locus.\n          <\/jats:p>","DOI":"10.1128\/aem.01187-17","type":"journal-article","created":{"date-parts":[[2017,8,26]],"date-time":"2017-08-26T00:25:41Z","timestamp":1503707141000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Mechanism for Restoration of Fertility in Hybrid Zygosaccharomyces rouxii Generated by Interspecies Hybridization"],"prefix":"10.1128","volume":"83","author":[{"given":"Jun","family":"Watanabe","sequence":"first","affiliation":[{"name":"Manufacturing Division, Yamasa Corporation, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenji","family":"Uehara","sequence":"additional","affiliation":[{"name":"Manufacturing Division, Yamasa Corporation, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoshinobu","family":"Mogi","sequence":"additional","affiliation":[{"name":"Manufacturing Division, Yamasa Corporation, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuichiro","family":"Tsukioka","sequence":"additional","affiliation":[{"name":"Manufacturing Division, Yamasa Corporation, Chiba, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.2012.0434"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/42711"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1095781"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02424"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002220"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1534\/genetics.111.134577"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04562"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.10.047"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.1002221"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.16.11.6110"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2012.00820.x"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8274-9"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.80.4.492-495.1960"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsyr.2005.02.004"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/yea.1598"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.fm.2012.02.005"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.043323-0"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/1567-1364.12027"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062121"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0152558"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.2002128"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/genomeA.01610-16"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dsp003"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1534\/g3.111.000745"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-13-407"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/dnares\/dst058"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.091546.109"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-13-237"},{"key":"e_1_3_3_30_2","volume-title":"Microbial genome annotation pipeline (MiGAP) for diverse users, S001-1-2","author":"Sugawara H","year":"2009","unstructured":"Sugawara H OhyamaA MoriH KurokawaK. 2009. 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