{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T18:41:28Z","timestamp":1778611288818,"version":"3.51.4"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A fermentative enrichment culture (designated DHM-1) was developed that is capable of cometabolically biotransforming high concentrations of chloroform (CF) to nontoxic end products. Two\n            <jats:italic>Pantoea<\/jats:italic>\n            spp. were isolated from DHM-1 that also possess this dechlorination capability. Following acclimation to increasing levels of CF, corn syrup-grown DHM-1 was able to transform over 500 mg\/liter CF in the presence of vitamin B\n            <jats:sub>12<\/jats:sub>\n            (approximately 3% of CF on a molar basis) at a rate as high as 22 mg\/liter\/day in a mineral salts medium. CO, CO\n            <jats:sub>2<\/jats:sub>\n            , and organic acids were the predominant biodegradation products, suggesting that hydrolytic reactions predominate during CF transformation. DHM-1 was capable of growing on corn syrup in the presence of high concentrations of CF (as may be present near contaminant source zones in groundwater), which makes it a promising culture for bioaugmentation. Strains DHM-1B and DHM-1T transform CF at rates similar to that of the DHM-1 enrichment culture. The ability of these strains to grow in the presence of high concentrations of CF appears to be related to alteration of membrane fluidity or homeoviscous and homeophasic adaptation.\n          <\/jats:p>","DOI":"10.1128\/aem.01191-10","type":"journal-article","created":{"date-parts":[[2010,8,7]],"date-time":"2010-08-07T04:00:31Z","timestamp":1281153631000},"page":"6463-6469","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Anaerobic Biotransformation of High Concentrations of Chloroform by an Enrichment Culture and Two Bacterial Isolates"],"prefix":"10.1128","volume":"76","author":[{"given":"Huifeng","family":"Shan","sequence":"first","affiliation":[{"name":"CH2M HILL, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Harry D.","family":"Kurtz","sequence":"additional","affiliation":[{"name":"Department of Genetics and Biochemistry, Clemson University, Clemson, South Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nadia","family":"Mykytczuk","sequence":"additional","affiliation":[{"name":"Department of Biology, Laurentian University, Sudbury, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack T.","family":"Trevors","sequence":"additional","affiliation":[{"name":"Department of Environmental Biology, Ontario Agricultural College, University of Guelph, Guelph, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David L.","family":"Freedman","sequence":"additional","affiliation":[{"name":"Department of Environmental Engineering and Earth Sciences, Clemson University, Clemson, South Carolina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2008.04.055"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.4.1031-1037.1991"},{"key":"e_1_3_2_5_2","unstructured":"ATSDR. October 2008 posting date. CERCLA priority list of hazardous substances. Agency for Toxic Substances and Disease Registry Atlanta GA. http:\/\/www.atsdr.cdc.gov\/cercla\/07list.html\n."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.9.3195-3201.1995"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(99)00121-9"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.72.121801.161828"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00060a027"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00258354"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.6.2726-2730.2002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(02)00155-0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.19.7843"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.54.11.2819-2824.1988"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10532-004-4877-9"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0956-053X(01)00032-0"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.57.10.2847-2857.1991"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.55.9.2144-2151.1989"},{"key":"e_1_3_2_19_2","first-page":"123","volume":"4","year":"1995","unstructured":"Freedman, D. L., M. Lasecki, S. Hashsham, and R. Scholze. 1995. Accelerated biotransformation of carbon tetrachloride and chloroform by sulfate-reducing enrichment cultures, p. 123-138. In R. E. Hinchee, A. Leeson, and L. Semprini (ed.), Bioremediation of chlorinated solvents,vol. 4. Battelle Press, Columbus, OH.","journal-title":"Bioremediation of chlorinated solvents,"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2009.02150.x"},{"key":"e_1_3_2_21_2","first-page":"1387","volume":"30","year":"1996","unstructured":"Gupta, M., A. Gupta, M. T. Suidan, and G. D. Sayles. 1996. Biotransformation rates of chloroform under anaerobic conditions. II. Sulfate reduction. Water Res.30:1387-1394.","journal-title":"II. Sulfate reduction. Water Res."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0043-1354(96)00005-X"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050776"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.10.4537-4542.1999"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/es00011a023"},{"key":"e_1_3_2_26_2","unstructured":"the 4th European Congress on Biotechnology 1987 vol. 3"},{"key":"e_1_3_2_27_2","unstructured":"Bacterial physiology and metabolism. 2008"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/jctb.632"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2001.tb10686.x"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2005.03.015"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1021\/es001419s"},{"key":"e_1_3_2_32_2","unstructured":"Brock biology of microorganisms 2003"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1021\/es001285i"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1985.tb00817.x"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01925-07"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.watres.2009.10.035"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.3.761-765.1996"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4157(78)90010-2"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.2.201-222.1995"},{"key":"e_1_3_2_40_2","first-page":"152","year":"1993","unstructured":"Stackebrandt, E., and W. Liesack. 1993. Nucleic acids and classification, p. 152-189. In M. Goodfellow and A. G. O'Donnell (ed.), Handbook of new bacterial systematics. Academic Press, London, England.","journal-title":"Handbook of new bacterial systematics"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-1878(199801)20:1<49::AID-BIES8>3.0.CO;2-N"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.139.1.39-47.1979"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.9.3304-3312.1996"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2004.03.005"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1021\/es990948x"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01191-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01191-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:18:18Z","timestamp":1645636698000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01191-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,10]]},"references-count":44,"journal-issue":{"issue":"19","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1128\/AEM.01191-10"],"URL":"https:\/\/doi.org\/10.1128\/aem.01191-10","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,10]]},"assertion":[{"value":"2010-05-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-08-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}