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Strain Q8r1-96 is representative of the D-genotype of 2,4-DAPG producers, which are exceptional because of their ability to aggressively colonize and maintain large populations on the roots of host plants, including wheat, pea, and sugar beet. In this study, three genes, an<jats:italic>sss<\/jats:italic>recombinase gene,<jats:italic>ptsP<\/jats:italic>, and<jats:italic>orfT<\/jats:italic>, which are important in the interaction of<jats:italic>Pseudomonas<\/jats:italic>spp. with various hosts, were investigated to determine their contributions to the unusual colonization properties of strain Q8r1-96. The<jats:italic>sss<\/jats:italic>recombinase and<jats:italic>ptsP<\/jats:italic>genes influence global processes, including phenotypic plasticity and organic nitrogen utilization, respectively. The<jats:italic>orfT<\/jats:italic>gene contributes to the pathogenicity of<jats:italic>Pseudomonas aeruginosa<\/jats:italic>in plants and animals and is conserved among saprophytic rhizosphere pseudomonads, but its function is unknown. Clones containing these genes were identified in a Q8r1-96 genomic library, sequenced, and used to construct gene replacement mutants of Q8r1-96. Mutants were characterized to determine their 2,4-DAPG production, motility, fluorescence, colony morphology, exoprotease and hydrogen cyanide (HCN) production, carbon and nitrogen utilization, and ability to colonize the rhizosphere of wheat grown in natural soil. The<jats:italic>ptsP<\/jats:italic>mutant was impaired in wheat root colonization, whereas mutants with mutations in the<jats:italic>sss<\/jats:italic>recombinase gene and<jats:italic>orfT<\/jats:italic>were not. However, all three mutants were less competitive than wild-type<jats:italic>P. fluorescens<\/jats:italic>Q8r1-96 in the wheat rhizosphere when they were introduced into the soil by paired inoculation with the parental strain.<\/jats:p>","DOI":"10.1128\/aem.01215-06","type":"journal-article","created":{"date-parts":[[2006,11,6]],"date-time":"2006-11-06T18:53:46Z","timestamp":1162839226000},"page":"7111-7122","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Role of<i>ptsP<\/i>,<i>orfT<\/i>, and<i>sss<\/i>Recombinase Genes in Root Colonization by<i>Pseudomonas fluorescens<\/i>Q8r1-96"],"prefix":"10.1128","volume":"72","author":[{"given":"Olga V.","family":"Mavrodi","sequence":"first","affiliation":[{"name":"Department of Plant Pathology, Washington State University, Pullman, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitri V.","family":"Mavrodi","sequence":"additional","affiliation":[{"name":"Department of Plant Pathology, Washington State University, Pullman, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Weller","sequence":"additional","affiliation":[{"name":"USDA-ARS, Root Disease and Biological Control Research Unit, Pullman, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linda S.","family":"Thomashow","sequence":"additional","affiliation":[{"name":"USDA-ARS, Root Disease and Biological Control Research Unit, Pullman, Washington"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1094\/MPMI.2004.17.8.872"},{"key":"e_1_3_2_3_2","doi-asserted-by":"crossref","unstructured":"Ausubel F. 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