{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,31]],"date-time":"2025-10-31T07:25:18Z","timestamp":1761895518908},"reference-count":25,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Desulfitobacterium hafniense<\/jats:named-content>\n            strain TCE1 is capable of metabolically reducing tetra- and trichloroethenes by organohalide respiration. A previous study revealed that the\n            <jats:italic>pce<\/jats:italic>\n            gene cluster responsible for this process is located on an active composite transposon, Tn-\n            <jats:italic>Dha1<\/jats:italic>\n            . In the present work, we investigated the effects on the stability of the transposon during successive subcultivations of strain TCE1 in a medium depleted of tetrachloroethene. At the physiological level, an increased fitness of the population was observed after 9 successive transfers and was correlated with a decrease in the level of production of the PceA enzyme. The latter observation was a result of the gradual loss of the\n            <jats:italic>pce<\/jats:italic>\n            genes in the population of strain TCE1 and not of a regulation mechanism, as was postulated previously for a similar phenomenon described for\n            <jats:named-content content-type=\"genus-species\">Sulfurospirillum multivorans<\/jats:named-content>\n            . A detailed molecular analysis of genetic rearrangements occurring around Tn-\n            <jats:italic>Dha1<\/jats:italic>\n            showed two independent but concomitant events, namely, the transposition of the first insertion sequence, IS\n            <jats:italic>Dha1<\/jats:italic>\n            -a, and homologous recombination across identical copies of IS\n            <jats:italic>Dha1<\/jats:italic>\n            flanking the transposon. A new model is proposed for the genetic heterogeneity around Tn-\n            <jats:italic>Dha1<\/jats:italic>\n            in\n            <jats:named-content content-type=\"genus-species\">D. hafniense<\/jats:named-content>\n            strain TCE1, along with some considerations for the cleavage mechanism mediated by the transposase TnpA1 encoded by IS\n            <jats:italic>Dha1<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/aem.01221-12","type":"journal-article","created":{"date-parts":[[2012,6,24]],"date-time":"2012-06-24T00:33:51Z","timestamp":1340498031000},"page":"6121-6127","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["The Physiological Opportunism of Desulfitobacterium hafniense Strain TCE1 towards Organohalide Respiration with Tetrachloroethene"],"prefix":"10.1128","volume":"78","author":[{"given":"Aur\u00e9lie","family":"Duret","sequence":"first","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne, School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christof","family":"Holliger","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne, School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julien","family":"Maillard","sequence":"additional","affiliation":[{"name":"Ecole Polytechnique F\u00e9d\u00e9rale de Lausanne, School of Architecture, Civil and Environmental Engineering, Laboratory for Environmental Biotechnology, Station 6, Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90061-7"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/tcr.20134"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-005-0112-9"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00979-06"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.12.5212-5221.1999"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01290-07"},{"key":"e_1_3_3_8_2","first-page":"115","volume-title":"Dehalogenation: microbial processes and environmental applications","author":"Holliger C","year":"2003","unstructured":"HolligerC RegeardC DiekertG. 2003. Dehalogenation by anaerobic bacteria, p 115\u2013157. In H\u00e4ggblomMM BossertIB (ed), Dehalogenation: microbial processes and environmental applications. 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