{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T00:02:01Z","timestamp":1791158521836,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2010,11,1]],"date-time":"2010-11-01T00:00:00Z","timestamp":1288569600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            is a Gram-positive, psychrotrophic, facultative intracellular food-borne pathogen responsible for severe illness (listeriosis). The bacteria can grow in a wide range of temperatures (1 to 45\u00b0C), and low-temperature growth contributes to the food safety hazards associated with contamination of ready-to-eat foods with this pathogen. To assess the impact of oxidative stress responses on the ability of\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            to grow at low temperatures and to tolerate repeated freeze-thaw stress (cryotolerance), we generated and characterized a catalase-deficient mutant of\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            F2365 harboring a\n            <jats:italic>mariner<\/jats:italic>\n            -based transposon insertion in the catalase gene (\n            <jats:italic>kat<\/jats:italic>\n            ). When grown aerobically on blood-free solid medium, the\n            <jats:italic>kat<\/jats:italic>\n            mutant exhibited impaired growth, with the extent of impairment increasing with decreasing temperature, and no growth was detected at 4\u00b0C. Aerobic growth in liquid was impaired at 4\u00b0C, especially under aeration, but not at higher temperatures (10, 25, or 37\u00b0C). Genetic complementation of the mutant with the intact\n            <jats:italic>kat<\/jats:italic>\n            restored normal growth, confirming that inactivation of this gene was responsible for the growth impairment. In spite of the expected impact of oxidative stress responses on cryotolerance, cryotolerance of the\n            <jats:italic>kat<\/jats:italic>\n            mutant was not affected.\n          <\/jats:p>","DOI":"10.1128\/aem.01223-10","type":"journal-article","created":{"date-parts":[[2010,9,4]],"date-time":"2010-09-04T02:34:25Z","timestamp":1283567665000},"page":"6998-7003","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Temperature-Dependent Requirement for Catalase in Aerobic Growth of\n            <i>Listeria monocytogenes<\/i>\n            F2365"],"prefix":"10.1128","volume":"76","author":[{"given":"Reha Onur","family":"Azizoglu","sequence":"first","affiliation":[{"name":"Department of Food, Bioprocessing and Nutrition Sciences, North Carolina State University, Raleigh, North 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