{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,12]],"date-time":"2026-03-12T01:11:17Z","timestamp":1773277877974,"version":"3.50.1"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["EXPL\/DTP-SAP\/2338\/2013"],"award-info":[{"award-number":["EXPL\/DTP-SAP\/2338\/2013"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["RECI\/AAG-TEC\/0400\/2012"],"award-info":[{"award-number":["RECI\/AAG-TEC\/0400\/2012"]}]},{"name":"Funda\u00e7\u00e3o para a Ci\u00eancia e Tecnologia","award":["UID\/Multi\/04349\/2013"],"award-info":[{"award-number":["UID\/Multi\/04349\/2013"]}]},{"DOI":"10.13039\/501100004493","name":"International Atomic Energy Agency","doi-asserted-by":"publisher","award":["Research Contract 17474"],"award-info":[{"award-number":["Research Contract 17474"]}],"id":[{"id":"10.13039\/501100004493","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Adenovirus is the most prevalent enteric virus in waters worldwide due to its environmental stability, which leads to public health concerns. Mitigation strategies are therefore required. The aim of this study was to assess the inactivation of human adenovirus type 5 (HAdV-5) by gamma radiation in aqueous environments. Various substrates with different organic loads, including domestic wastewater, were inoculated with HAdV-5 either individually or in a viral pool (with murine norovirus type 1 [MNV-1]) and were irradiated in a Cobalt-60 irradiator at several gamma radiation doses (0.9 to 10.8 kGy). The infectivity of viral particles, before and after irradiation, was tested by plaque assay using A549 cells. D\n            <jats:sub>10<\/jats:sub>\n            values (dose required to inactivate 90% of a population or the dose of irradiation needed to produce a 1 log\n            <jats:sub>10<\/jats:sub>\n            reduction in the population) were estimated for each substrate based on virus infectivity inactivation exponential kinetics. The capability of two detection methods, nested PCR and enzyme-linked immunosorbent assay (ELISA), to track inactivated viral particles was also assessed. After irradiation at 3.5 kGy, a reduction of the HAdV-5 titer of 4 log PFU\/ml on substrates with lower organic loads was obtained, but in highly organic matrixes, the virus titer reduction was only 1 log PFU\/ml. The D\n            <jats:sub>10<\/jats:sub>\n            values of HAdV-5 in high organic substrates were significantly higher than in water suspensions. The obtained results point out some discrepancies between nested PCR, ELISA, and plaque assay on the assessments of HAdV-5 inactivation. These results suggest that the inactivation of HAdV-5 by gamma radiation, in aqueous environments, is significantly affected by substrate composition. This study highlights the virucidal potential of gamma radiation that may be used as a disinfection treatment for sustainable water supplies.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Human adenovirus (HAdV) is the most prevalent of the enteric viruses in environmental waters worldwide. The purposes of this study are to provide new insights on the inactivation of enteric virus by gamma irradiation and to introduce new concepts and reinforce the benefits and utility of radiation technologies as disinfection processes. This may be an effective tool to guarantee the reduction of viral pathogens and to contribute to public health and sustainable water supplies.\n          <\/jats:p>","DOI":"10.1128\/aem.01229-16","type":"journal-article","created":{"date-parts":[[2016,6,18]],"date-time":"2016-06-18T02:23:51Z","timestamp":1466216631000},"page":"5166-5173","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["Tracking Human Adenovirus Inactivation by Gamma Radiation under Different Environmental Conditions"],"prefix":"10.1128","volume":"82","author":[{"given":"Andreia I.","family":"Pimenta","sequence":"first","affiliation":[{"name":"Centro de Ci\u00eancias e Tecnologias Nucleares (C2TN), Instituto Superior T\u00e9cnico, Universidade de Lisboa, Bobadela, Loures, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Duarte","family":"Guerreiro","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias e Tecnologias Nucleares (C2TN), Instituto Superior T\u00e9cnico, Universidade de Lisboa, Bobadela, Loures, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joana","family":"Madureira","sequence":"additional","affiliation":[{"name":"Centro de Ci\u00eancias e Tecnologias Nucleares (C2TN), Instituto Superior T\u00e9cnico, Universidade de Lisboa, Bobadela, Loures, Portugal"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernanda M. 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