{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,21]],"date-time":"2026-05-21T05:15:51Z","timestamp":1779340551781,"version":"3.51.4"},"reference-count":86,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2016,8,15]],"date-time":"2016-08-15T00:00:00Z","timestamp":1471219200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2016,9,1]],"date-time":"2016-09-01T00:00:00Z","timestamp":1472688000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Endosymbiosis is a unique form of interaction between organisms, with one organism dwelling inside the other. One of the most widespread endosymbionts is<jats:named-content content-type=\"genus-species\">Wolbachia pipientis<\/jats:named-content>, a maternally transmitted bacterium carried by insects, crustaceans, mites, and filarial nematodes. Although candidate proteins that contribute to maternal transmission have been identified, the molecular basis for maternal<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>transmission remains largely unknown. To investigate transmission-related processes in response to<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>infection, ovarian proteomes were analyzed from<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>-infected<jats:named-content content-type=\"genus-species\">Drosophila melanogaster<\/jats:named-content>and<jats:named-content content-type=\"genus-species\">D. simulans.<\/jats:named-content>Endogenous and variant host-strain combinations were investigated. Significant and differentially abundant ovarian proteins were detected, indicating substantial regulatory changes in response to<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>. Variant<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>strains were associated with a broader impact on the ovary proteome than endogenous<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>strains. The<jats:named-content content-type=\"genus-species\">D. melanogaster<\/jats:named-content>ovarian environment also exhibited a higher level of diversity of proteomic responses to<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>than<jats:named-content content-type=\"genus-species\">D. simulans.<\/jats:named-content>Overall, many<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>-responsive ovarian proteins detected in this study were consistent with expectations from the experimental literature. This suggests that context-specific changes in protein abundance contribute to<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>manipulation of transmission-related mechanisms in oogenesis.<\/jats:p><jats:p><jats:bold>IMPORTANCE<\/jats:bold>Millions of insect species naturally carry bacterial endosymbionts called<jats:named-content content-type=\"genus-species\">Wolbachia. Wolbachia<\/jats:named-content>bacteria are transmitted by females to their offspring through a robust egg-loading mechanism. The molecular basis for<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>transmission remains poorly understood at this time, however. This proteomic study identified specific fruit fly ovarian proteins as being upregulated or downregulated in response to<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>infection. The majority of these protein responses correlated specifically with the type of host and<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>strain involved. This work corroborates previously identified factors and mechanisms while also framing the broader context of ovarian manipulation by<jats:named-content content-type=\"genus-species\">Wolbachia<\/jats:named-content>.<\/jats:p>","DOI":"10.1128\/aem.01255-16","type":"journal-article","created":{"date-parts":[[2016,6,26]],"date-time":"2016-06-26T06:02:12Z","timestamp":1466920932000},"page":"5354-5363","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Wolbachia Endosymbionts Modify Drosophila Ovary Protein Levels in a Context-Dependent Manner"],"prefix":"10.1128","volume":"82","author":[{"given":"Steen","family":"Christensen","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Biomolecular Sciences Institute, Florida International University, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"P\u00e9rez Dulzaides","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Biomolecular Sciences Institute, Florida International University, Miami, Florida, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Victoria E.","family":"Hedrick","sequence":"additional","affiliation":[{"name":"Bindley Bioscience Center, Purdue Proteomics Facility, Purdue University, West Lafayette, Indiana, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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