{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T14:00:01Z","timestamp":1790172001382,"version":"4.1.0"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"USDA","award":["5040-12630-007-000-D"],"award-info":[{"award-number":["5040-12630-007-000-D"]}],"id":[{"id":"https:\/\/ror.org\/01na82s61","id-type":"ROR","asserted-by":"crossref"}]},{"name":"USDA","award":["5040-12630-007-041-A"],"award-info":[{"award-number":["5040-12630-007-041-A"]}],"id":[{"id":"https:\/\/ror.org\/01na82s61","id-type":"ROR","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,9,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      In two randomized controlled field studies, we investigated the effect of biochar-amended dairy manure composting on the concentrations and prevalence of generic and antibiotic-resistant\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      and enterococci. Fresh dairy manure mixed with sawdust and biochar was randomly distributed into plastic reactors and composted for 6 months. Pre-compost (biochar, sawdust, fresh dairy manure), manure mixture, and final compost samples were cultured for enumeration and prevalence. While biochar and sawdust were not significant sources, fresh dairy manure was the major contributor of bacteria to the initial manure mixture. Biochar amendment did not significantly reduce bacterial load beyond that could be achieved by composting alone. Spring-summer composting was more effective than fall-winter composting. While\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      was more prone, enterococci were more likely to survive composting. Dairy manure composting eliminated bacteria (3rd generation cephalosporin-resistant [3GC\n                      <jats:sup>r<\/jats:sup>\n                      ] and extended spectrum beta-lactamase-producing [ESBL]-\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      ) resistant to critically important and highest priority antibiotics for human health and significantly reduced the prevalence and concentrations of other investigated bacteria. 3GC\n                      <jats:sup>r<\/jats:sup>\n                      - and ESBL-\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      from fresh dairy manure were multidrug resistant and carried\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M<\/jats:sub>\n                      genes, a widely distributed ESBL gene type. By whole-genome sequencing, ESBL-\n                      <jats:italic toggle=\"yes\">E. coli<\/jats:italic>\n                      from experiment 1 (fall-winter) had diverse resistance profiles, sequence types, plasmid profiles, virulence factor genes, and more\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M<\/jats:sub>\n                      genes (CTX-M-1, CTX-M-32, and CTX-M-55). However, those from experiment 2 (spring-summer) were clonal and carried\n                      <jats:italic toggle=\"yes\">bla<\/jats:italic>\n                      <jats:sub>CTX-M-55<\/jats:sub>\n                      only. In conclusion, dairy manure composting effectively removes antibiotic resistant bacteria of public health importance, with no added effect of biochar amendment.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>Composting is a microbially mediated decomposition of organic matter into a nutrient-rich product for use as fertilizer. Biochar, obtained from the pyrolysis of biomass including animal manure, has been used for soil amendment. Beyond its nutrient stabilizing potential, composting has been shown to reduce bacterial load in animal manure. However, its effect with or without a biochar addition was not evaluated under a randomized controlled field trial. Biochar was nearly bacteriologically \u201csterile,\u201d and the sawdust used as a bulking agent had low concentrations of innocuous bacteria indicating that dairy manure was the main source of bacteria to the pre-compost mixture. Biochar did not have an extra effect on the bacterial load beyond composting alone. Composting during spring\/summer is more effective than fall\/winter. Furthermore, it is more effective against gram-negatives than gram-positive bacteria. Importantly, composting effectively eliminated multidrug-resistant bacteria of critical importance to public health for safer use of dairy manure.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01258-26","type":"journal-article","created":{"date-parts":[[2026,8,13]],"date-time":"2026-08-13T13:00:17Z","timestamp":1786626017000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Composting dairy cattle manure significantly reduces antibiotic-resistant bacteria with no biochar impact"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9903-5283","authenticated-orcid":true,"given":"Getahun E.","family":"Agga","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01na82s61","id-type":"ROR","asserted-by":"publisher"}],"name":"Food Animal Environmental Systems Research Unit, Agricultural Research Service, U.S. Department of Agriculture","place":["Bowling Green, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annesly","family":"Netthisinghe","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0446vnd56","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Agriculture and Food Science, Western Kentucky University","place":["Bowling Green, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Woosley","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0446vnd56","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Agriculture and Food Science, Western Kentucky University","place":["Bowling Green, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William","family":"Strunk","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0446vnd56","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Agriculture and Food Science, Western Kentucky University","place":["Bowling Green, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karamat R.","family":"Sistani","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01na82s61","id-type":"ROR","asserted-by":"publisher"}],"name":"Food Animal Environmental Systems Research Unit, Agricultural Research Service, U.S. Department of Agriculture","place":["Bowling Green, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_4_2_2","doi-asserted-by":"publisher","DOI":"10.3168\/jds.2019-16778"},{"key":"e_1_3_4_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jeq2.20560"},{"key":"e_1_3_4_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.envpol.2025.126531"},{"key":"e_1_3_4_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.scitotenv.2025.180306"},{"key":"e_1_3_4_6_2","doi-asserted-by":"publisher","DOI":"10.3390\/antibiotics11030391"},{"key":"e_1_3_4_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhazmat.2020.123634"},{"key":"e_1_3_4_8_2","doi-asserted-by":"publisher","DOI":"10.3390\/agronomy14050952"},{"key":"e_1_3_4_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00750-21"},{"key":"e_1_3_4_10_2","doi-asserted-by":"publisher","DOI":"10.3390\/app9173494"},{"key":"e_1_3_4_11_2","volume-title":"Mastitis in dairy cattle, sheep and goats","author":"Gelalcha BD","year":"2021","unstructured":"GelalchaBD, AggaGE, DegoOK. 2021. 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