{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:44:43Z","timestamp":1788245083531,"version":"build-2803163510"},"reference-count":36,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2010,10,1]],"date-time":"2010-10-01T00:00:00Z","timestamp":1285891200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The genotyping of numerous isolates of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            parasites has led to the definition of new species and a better understanding of the epidemiology of cryptosporidiosis. A single-locus genotyping method based on the partial sequence of a polymorphic sporozoite surface glycoprotein gene (\n            <jats:italic>GP60<\/jats:italic>\n            ) has been favored by many for surveying\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            and\n            <jats:italic>C. hominis<\/jats:italic>\n            populations. Since genetically distinct\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            parasites recombine in nature, it is unclear whether single-locus classifications can adequately represent intraspecies diversity. To address this question, we investigated whether multilocus genotypes of\n            <jats:italic>C. parvum<\/jats:italic>\n            and\n            <jats:italic>C. hominis<\/jats:italic>\n            cluster according to the\n            <jats:italic>GP60<\/jats:italic>\n            genotype.\n            <jats:italic>C. hominis<\/jats:italic>\n            multilocus genotypes did not segregate according to this marker, indicating that for this species the\n            <jats:italic>GP60<\/jats:italic>\n            sequence is not a valid surrogate for multilocus typing methods. In contrast, in\n            <jats:italic>C. parvum<\/jats:italic>\n            the previously described \u201canthroponotic\u201d genotype was confirmed as a genetically distinct subspecies cluster characterized by a diagnostic GP60 allele. However, as in\n            <jats:italic>C. hominis<\/jats:italic>\n            , several\n            <jats:italic>C. parvum<\/jats:italic>\n            GP60 alleles did not correlate with distinct subpopulations. Given the rarity of some\n            <jats:italic>C. parvum<\/jats:italic>\n            GP60 alleles in our sample, the existence of additional\n            <jats:italic>C. parvum<\/jats:italic>\n            subgroups with unique GP60 alleles cannot be ruled out. We conclude that with the exception of genotypically distinct\n            <jats:italic>C. parvum<\/jats:italic>\n            subgroups, multilocus genotyping methods are needed to characterize\n            <jats:italic>C. parvum<\/jats:italic>\n            and\n            <jats:italic>C. hominis<\/jats:italic>\n            populations. Unless parasite virulence is controlled at the GP60 locus, attempts to find associations within species or subspecies between GP60 and phenotype are unlikely to be successful.\n          <\/jats:p>","DOI":"10.1128\/aem.01268-10","type":"journal-article","created":{"date-parts":[[2010,8,14]],"date-time":"2010-08-14T01:57:24Z","timestamp":1281751044000},"page":"6639-6644","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":54,"title":["Comparison of Single- and Multilocus Genetic Diversity in the Protozoan Parasites\n            <i>Cryptosporidium parvum<\/i>\n            and\n            <i>C. hominis<\/i>"],"prefix":"10.1128","volume":"76","author":[{"given":"Giovanni","family":"Widmer","sequence":"first","affiliation":[{"name":"Tufts Cummings School of Veterinary Medicine, Division of Infectious Diseases, 200 Westboro Road, North Grafton, Massachusetts 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