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The enzyme is only known to be produced by a small subset of microorganisms, although thiaminase I activity has been associated with numerous plants and animals, and is implicated in thiamine deficiencies brought on by consumption of organisms containing this enzyme. Genomic and biochemical analyses have shed light on potential roles for the enzyme. Using the genetically amenable thiaminase I-producing soil bacterium<jats:named-content content-type=\"genus-species\">Burkholderia thailandensis<\/jats:named-content>, we were able to demonstrate that thiaminase I helps salvage precursors from thiamine derivatives in the environment and degrades thiamine to its precursors, which are preferentially used by<jats:named-content content-type=\"genus-species\">B. thailandensis<\/jats:named-content>auxotrophs. Our study establishes a biological role for this perplexing enzyme and provides insight into the complicated nature of thiamine metabolism. It also establishes<jats:named-content content-type=\"genus-species\">B. thailandensis<\/jats:named-content>as a robust model system for studying thiamine metabolism.<\/jats:p>","DOI":"10.1128\/aem.01268-18","type":"journal-article","created":{"date-parts":[[2018,7,12]],"date-time":"2018-07-12T10:08:33Z","timestamp":1531390113000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Thiaminase I Provides a Growth Advantage by Salvaging Precursors from Environmental Thiamine and Its Analogs in Burkholderia thailandensis"],"prefix":"10.1128","volume":"84","author":[{"given":"David R.","family":"Sannino","sequence":"first","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clifford E.","family":"Kraft","sequence":"additional","affiliation":[{"name":"Department of Natural Resources, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katie A.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4787-5264","authenticated-orcid":false,"given":"Esther R.","family":"Angert","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmicrobiol.2016.213"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/692168"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.biochem.78.072407.102340"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01636-08"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.15.8946"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.11.3660-3662.2004"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00641-06"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2014.61"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01208-08"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2004.08.037"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M208965200"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1021\/acschembio.5b00918"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi0478648"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1107\/S0907444905005147"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1567-1364.2007.00333.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20140522"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio.2007.13"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1315882110"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.7.3445"},{"key":"e_1_3_3_21_2","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1002\/9780470122600.ch9","article-title":"Thiaminase","volume":"15","author":"Fujita A","year":"1954","unstructured":"Fujita A. 1954. 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