{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T09:29:30Z","timestamp":1791278970971,"version":"4.1.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2006,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>1,1,1-Trichloroethane (1,1,1-TCA) is a common groundwater pollutant as a result of improper disposal and accidental spills. It is often found as a cocontaminant with trichloroethene (TCE) and inhibits some TCE-degrading microorganisms. 1,1,1-TCA removal is therefore required for effective bioremediation of sites contaminated with mixed chlorinated organics. This study characterized MS, a 1,1,1-TCA-degrading, anaerobic, mixed microbial culture derived from a 1,1,1-TCA-contaminated site in the northeastern United States. MS reductively dechlorinated 1,1,1-TCA to 1,1-dichloroethane (1,1-DCA) and then to monochloroethane (CA) but not further. Cloning of bacterial 16S rRNA genes revealed among other organisms the presence of a<jats:italic>Dehalobacter<\/jats:italic>sp. and a<jats:italic>Desulfovibrio<\/jats:italic>sp., which are both phylogenetically related to known dehalorespiring strains. Monitoring of these populations with species-specific quantitative PCR during degradation of 1,1,1-TCA and 1,1-DCA showed that<jats:italic>Dehalobacter<\/jats:italic>proliferated during dechlorination.<jats:italic>Dehalobacter<\/jats:italic>growth was dechlorination dependent, whereas<jats:italic>Desulfovibrio<\/jats:italic>growth was dechlorination independent. Experiments were also performed to test whether MS could enhance TCE degradation in the presence of inhibiting levels of 1,1,1-TCA. Dechlorination of<jats:italic>cis<\/jats:italic>-dichloroethene (cDCE) and vinyl chloride (VC) in KB-1, a chloroethene-degrading culture used for bioaugmentation, was inhibited with 1,1,1-TCA present. When KB-1 and MS were coinoculated, degradation of cDCE and VC to ethene proceeded as soon as the 1,1,1-TCA was dechlorinated to 1,1-DCA by MS. This demonstrated the potential application of the MS and KB-1 cultures for cobioaugmentation of sites cocontaminated with 1,1,1-TCA and TCE.<\/jats:p>","DOI":"10.1128\/aem.01269-06","type":"journal-article","created":{"date-parts":[[2006,12,5]],"date-time":"2006-12-05T18:31:39Z","timestamp":1165343499000},"page":"7849-7856","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":99,"title":["A 1,1,1-Trichloroethane-Degrading Anaerobic Mixed Microbial Culture Enhances Biotransformation of Mixtures of Chlorinated Ethenes and Ethanes"],"prefix":"10.1128","volume":"72","author":[{"given":"Ariel","family":"Grostern","sequence":"first","affiliation":[{"name":"Department of Cell and Systems Biology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth A.","family":"Edwards","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Applied Chemistry, University of Toronto, Toronto, Ontario, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0043-1354(98)00344-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1021\/es990809f"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/35046063"},{"key":"e_1_3_2_5_2","unstructured":"ATSDR. 2004. ToxFAQs for 1 1 1-trichloroethane agency for toxic substances and disease registry. [Online.] http:\/\/www.atsdr.cdc.gov\/tfacts70.html . 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