{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T14:44:20Z","timestamp":1790433860776,"version":"4.1.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2017,10,1]],"date-time":"2017-10-01T00:00:00Z","timestamp":1506816000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000005","name":"Department of Defense","doi-asserted-by":"crossref","award":["DM110308"],"award-info":[{"award-number":["DM110308"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Allen School"},{"name":"Libyan Ministry of Higher Education"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2017,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Biofilm-associated infections are a clinical challenge, in part because a hydrated matrix protects the bacterial community from antibiotics. Herein, we evaluated how different osmotic compounds (maltodextrin, sucrose, and polyethylene glycol [PEG]) enhance antibiotic efficacy against\n            <jats:named-content content-type=\"genus-species\">Acinetobacter baumannii<\/jats:named-content>\n            biofilm communities. Established (24-h) test tube biofilms (strain ATCC 17978) were treated with osmotic compounds in the presence or absence of 10\u00d7 the MIC of different antibiotics (50 \u03bcg\/ml tobramycin, 20 \u03bcg\/ml ciprofloxacin, 300 \u03bcg\/ml chloramphenicol, 30 \u03bcg\/ml nalidixic acid, or 100 \u03bcg\/ml erythromycin). Combining antibiotics with hypertonic concentrations of the osmotic compounds for 24 h reduced the number of biofilm bacteria by 5 to 7 log (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). Increasing concentrations of osmotic compounds improved the effect, but there was a trade-off with increasing solution viscosity, whereby low-molecular-mass compounds (sucrose, 400-Da PEG) worked better than higher-mass compounds (maltodextrin, 3,350-Da PEG). Ten other\n            <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n            strains were similarly treated with 400-Da PEG and tobramycin, resulting in a mean 2.7-log reduction in recoverable bacteria compared with tobramycin treatment alone. Multivariate regression models with data from different osmotic compounds and nine antibiotics demonstrated that the benefit from combining hypertonic treatments with antibiotics is a function of antibiotic mass and lipophilicity (\n            <jats:italic>r<\/jats:italic>\n            <jats:sup>\n              <jats:italic>2<\/jats:italic>\n            <\/jats:sup>\n            &gt; 0.82;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.002), and the relationship was generalizable for biofilms formed by\n            <jats:named-content content-type=\"genus-species\">A. baumannii<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            K-12. Augmenting topical antibiotic therapies with a low-mass hypertonic treatment may enhance the efficacy of antibiotics against wound biofilms, particularly when using low-mass hydrophilic antibiotics.\n          <\/jats:p>\n          <jats:p>\n            <jats:bold>IMPORTANCE<\/jats:bold>\n            Biofilms form a barrier that protects bacteria from environmental insults, including exposure to antibiotics. We demonstrated that multiple osmotic compounds can enhance antibiotic efficacy against\n            <jats:named-content content-type=\"genus-species\">Acinetobacter baumannii<\/jats:named-content>\n            biofilm communities, but viscosity is a limiting factor, and the most effective compounds have lower molecular mass. The synergism between osmotic compounds and antibiotics is also dependent on the hydrophobicity and mass of the antibiotics. The statistical models presented herein provide a basis for predicting the optimal combination of osmotic compounds and antibiotics against surface biofilms communities.\n          <\/jats:p>","DOI":"10.1128\/aem.01297-17","type":"journal-article","created":{"date-parts":[[2017,7,22]],"date-time":"2017-07-22T00:40:23Z","timestamp":1500684023000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Osmotic Compounds Enhance Antibiotic Efficacy against Acinetobacter baumannii Biofilm Communities"],"prefix":"10.1128","volume":"83","author":[{"given":"Azeza","family":"Falghoush","sequence":"first","affiliation":[{"name":"Paul G. 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