{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:05:12Z","timestamp":1762160712401},"reference-count":28,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2009,1,1]],"date-time":"2009-01-01T00:00:00Z","timestamp":1230768000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Effective management of human cryptosporidiosis requires efficient methods for detection and identification of the species of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            isolates. Identification of isolates to the species level is not routine for diagnostic assessment of cryptosporidiosis, which leads to uncertainty about the epidemiology of the\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            species that cause human disease. We developed a rapid and reliable method for species identification of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            oocysts from human fecal samples using terminal restriction fragment polymorphism (T-RFLP) analysis of the 18S rRNA gene. This method generated diagnostic fragments unique to the species of interest. A panel of previously identified isolates of species was blind tested to validate the method, which determined the correct species identity in every case. The T-RFLP profiles obtained for samples spiked with known amounts of\n            <jats:italic>Cryptosporidium hominis<\/jats:italic>\n            and\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            oocysts generated the two expected diagnostic peaks. The detection limit for an individual species was 1% of the total DNA. This is the first application of T-RFLP to protozoa, and the method which we developed is a rapid, repeatable, and cost-effective method for species identification.\n          <\/jats:p>","DOI":"10.1128\/aem.01341-08","type":"journal-article","created":{"date-parts":[[2008,11,1]],"date-time":"2008-11-01T00:56:19Z","timestamp":1225500979000},"page":"108-112","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":25,"title":["Terminal Restriction Fragment Length Polymorphism for Identification of\n            <i>Cryptosporidium<\/i>\n            Species in Human Feces"],"prefix":"10.1128","volume":"75","author":[{"given":"L. S.","family":"Waldron","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B. C.","family":"Ferrari","sequence":"additional","affiliation":[{"name":"Chemistry and Biomolecular Sciences, Macquarie University, Sydney, New South Wales 2109, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. R.","family":"Gillings","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. L.","family":"Power","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.10.5765-5771.2003"},{"key":"e_1_3_3_3_2","first-page":"241","volume":"30","year":"1998","unstructured":"Baveja, U. K. 1998. Acid fast staining versus ELISA for detection of Cryptosporidium in stool. 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