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The effector proteins exported by<jats:named-content content-type=\"genus-species\">Neospora<\/jats:named-content>induce its pathogenesis by modulating the host immunity. We show that most of the transcriptomic effects in<jats:named-content content-type=\"genus-species\">N. caninum<\/jats:named-content>-infected cells depend upon the activity of NcGRA7. A deficiency in NcGRA7 reduced the virulence of the parasite in mice. 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