{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:52:40Z","timestamp":1770220360928,"version":"3.49.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2015,8,15]],"date-time":"2015-08-15T00:00:00Z","timestamp":1439596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cats are considered the main reservoir of three zoonotic\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            species:\n            <jats:named-content content-type=\"genus-species\">Bartonella henselae<\/jats:named-content>\n            ,\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            <jats:named-content content-type=\"genus-species\">clarridgeiae<\/jats:named-content>\n            , and\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            <jats:named-content content-type=\"genus-species\">koehlerae<\/jats:named-content>\n            . Cat fleas (\n            <jats:named-content content-type=\"genus-species\">Ctenocephalides felis<\/jats:named-content>\n            ) have been experimentally demonstrated to be a competent vector of\n            <jats:named-content content-type=\"genus-species\">B. henselae<\/jats:named-content>\n            and have been proposed as the potential vector of the two other\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            species. Previous studies have reported a lack of association between the\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            species infection status (infected or uninfected) and\/or bacteremia levels of cats and the infection status of the fleas they host. Nevertheless, to date, no study has compared the quantitative distributions of these bacteria in both cats and their fleas under natural conditions. Thus, the present study explored these relationships by identifying and quantifying the different\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            species in both cats and their fleas. Therefore, EDTA-blood samples and fleas collected from stray cats were screened for\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            bacteria. Bacterial loads were quantified by high-resolution melt real-time quantitative PCR assays. The results indicated a moderate correlation between the\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            bacterial loads in the cats and their fleas when both were infected with the same\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            species. Moreover, a positive effect of the host infection status on the\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            bacterial loads of the fleas was observed. Conversely, the cat bacterial loads were not affected by the infection status of their fleas. Our results suggest that the\n            <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n            bacterial loads of fleas are positively affected by the presence of the bacteria in their feline host, probably by multiple acquisitions\/accumulation and\/or multiplication events.\n          <\/jats:p>","DOI":"10.1128\/aem.01370-15","type":"journal-article","created":{"date-parts":[[2015,6,13]],"date-time":"2015-06-13T03:30:53Z","timestamp":1434166253000},"page":"5613-5621","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Relationship between the Presence of Bartonella Species and Bacterial Loads in Cats and Cat Fleas (Ctenocephalides felis) under Natural Conditions"],"prefix":"10.1128","volume":"81","author":[{"given":"Ricardo","family":"Guti\u00e9rrez","sequence":"first","affiliation":[{"name":"Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaarit","family":"Nachum-Biala","sequence":"additional","affiliation":[{"name":"Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shimon","family":"Harrus","sequence":"additional","affiliation":[{"name":"Koret School of Veterinary Medicine, The Hebrew University of Jerusalem, Rehovot, Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1209"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2010.04679.x"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.9.2800-2800.1998"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.7.1813-1818.1997"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/514671"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.8.2943-2948.2000"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3462-3468.2004"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2009.08.005"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(02)00175-X"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.2.466-474.2002"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.8.1952-1956.1996"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1108.050101"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01451-06"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.2460\/javma.2004.224.1270"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.2009.0372.x"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2011.04.005"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0463.2002.100405.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1469-0691.2002.00434.x"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.593"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1051\/vetres:2005009"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(98)00153-6"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0147-9571(96)00025-2"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2012.10.004"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2013.1308"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1171-1176.2005"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-4-891"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.7.1797-1803.1995"},{"key":"e_1_3_3_29_2","first-page":"379","article-title":"Species Identification of feline DNA based on analysis of cytochrome b","volume":"9","author":"Natonek-Wisniewska M","year":"2009","unstructured":"Natonek-Wisniewska M. 2009. 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