{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T02:28:05Z","timestamp":1791599285496,"version":"4.3.4"},"reference-count":54,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2015,9,15]],"date-time":"2015-09-15T00:00:00Z","timestamp":1442275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The high morbidity and mortality in pigeons caused by pigeon paramyxovirus type 1 (PPMV-1) highlights the need for new insights into the host immune response and novel treatment approaches. Host defense peptides (HDPs) are key components of the innate immune system. In this study, three novel avian \u03b2-defensins (AvBDs 2, 7, and 10) were characterized in pigeons and shown to possess direct antiviral activity against PPMV-1\n            <jats:italic>in vitro<\/jats:italic>\n            . In addition, we evaluated the mRNA expression of these AvBDs and other immune-related genes in tissues of 2-month-old infected pigeons at 3 and 7 days postinfection. We observed that the expression of AvBD2 in the cecal tonsil, lungs, and proventriculus, as well as the expression of AvBD10 in the spleen, lungs, proventriculus, and kidneys, was upregulated in infected pigeons. Similarly, the expression of both Toll-like receptor 3 (TLR3) and TLR7 was increased in the spleen, trachea, and proventriculus, while TLR15 expression was increased only in the lungs of infected pigeons. In addition, inducible nitric oxide synthase (iNOS) expression was upregulated in the spleen, the bursa of Fabricius, the trachea, and the proventriculus of infected pigeons. Furthermore, we observed a high correlation between the expression of AvBD2 and the expression of either TLR7 or TLR15, as well as between AvBD10 expression and either TLR3 or TLR7 expression in respective tissues. The results suggest that PPMV-1 infection can induce innate host responses characterized by the activation of TLRs, particularly TLR3 and TLR7, AvBDs (2 and 10), and iNOS in pigeons.\n          <\/jats:p>","DOI":"10.1128\/aem.01413-15","type":"journal-article","created":{"date-parts":[[2015,7,11]],"date-time":"2015-07-11T02:45:59Z","timestamp":1436582759000},"page":"6415-6424","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Host Avian Beta-Defensin and Toll-Like Receptor Responses of Pigeons following Infection with Pigeon Paramyxovirus Type 1"],"prefix":"10.1128","volume":"81","author":[{"given":"Yanyan","family":"Li","sequence":"first","affiliation":[{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin, People's Republic of China"},{"name":"Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qianqian","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin, People's Republic of China"},{"name":"Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tingting","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin, People's Republic of China"},{"name":"Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengying","family":"Gao","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin, People's Republic of China"},{"name":"Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiuling","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin, People's Republic of China"},{"name":"Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zongxi","family":"Han","sequence":"additional","affiliation":[{"name":"Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhao","family":"Shao","sequence":"additional","affiliation":[{"name":"Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deying","family":"Ma","sequence":"additional","affiliation":[{"name":"College of Animal Science and Technology, Northeast Agricultural University, Harbin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengwang","family":"Liu","sequence":"additional","affiliation":[{"name":"Division of Avian Infectious Diseases, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People's Republic of China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2013.09.015"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/vir.0.026344-0"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.143.8.209"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1637\/7618-042606R1.1"},{"key":"e_1_3_2_6_2","first-page":"64","volume-title":"Diseases of poultry","author":"Alexander DJ","year":"2003","unstructured":"Alexander DJ. 2003. 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