{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T02:10:12Z","timestamp":1792203012779,"version":"4.4.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"17","license":[{"start":{"date-parts":[[2012,9,1]],"date-time":"2012-09-01T00:00:00Z","timestamp":1346457600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The Rieske nonheme mononuclear iron oxygenase MdpJ of the fuel oxygenate-degrading bacterial strain\n            <jats:named-content content-type=\"genus-species\">Aquincola tertiaricarbonis<\/jats:named-content>\n            L108 has been described to attack short-chain tertiary alcohols via hydroxylation and desaturation reactions. Here, we demonstrate that also short-chain secondary alcohols can be transformed by MdpJ. Wild-type cells of strain L108 converted 2-propanol and 2-butanol to 1,2-propanediol and 3-buten-2-ol, respectively, whereas an\n            <jats:italic>mdpJ<\/jats:italic>\n            knockout mutant did not show such activity. In addition, wild-type cells converted 3-methyl-2-butanol and 3-pentanol to the corresponding desaturation products 3-methyl-3-buten-2-ol and 1-penten-3-ol, respectively. The enzymatic hydroxylation of 2-propanol resulted in an enantiomeric excess of about 70% for the (\n            <jats:italic>R<\/jats:italic>\n            )-enantiomer, indicating that this reaction was favored. Likewise, desaturation of (\n            <jats:italic>R<\/jats:italic>\n            )-2-butanol to 3-buten-2-ol was about 2.3-fold faster than conversion of the (\n            <jats:italic>S<\/jats:italic>\n            )-enantiomer. The biotechnological potential of MdpJ for the synthesis of enantiopure short-chain alcohols and diols as building block chemicals is discussed.\n          <\/jats:p>","DOI":"10.1128\/aem.01434-12","type":"journal-article","created":{"date-parts":[[2012,6,30]],"date-time":"2012-06-30T03:07:40Z","timestamp":1341025660000},"page":"6280-6284","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Synthesis of Short-Chain Diols and Unsaturated Alcohols from Secondary Alcohol Substrates by the Rieske Nonheme Mononuclear Iron Oxygenase MdpJ"],"prefix":"10.1128","volume":"78","author":[{"given":"Franziska","family":"Sch\u00e4fer","sequence":"first","affiliation":[{"name":"Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Schuster","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"W\u00fcrz","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claus","family":"H\u00e4rtig","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hauke","family":"Harms","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland H.","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thore","family":"Rohwerder","sequence":"additional","affiliation":[{"name":"Helmholtz Centre for Environmental Research, Department of Environmental Microbiology, Leipzig, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.3.1180-1185.1999"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10532-011-9455-3"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1039\/p19930000399"},{"key":"e_1_3_3_5_2","first-page":"370","article-title":"Elucidating MTBE degradation in a mixed consortium using a multidisciplinary approach","volume":"73","author":"Bastida F","year":"2010","unstructured":"BastidaF. 2010. Elucidating MTBE degradation in a mixed consortium using a multidisciplinary approach. FEMS Microbiol. Ecol. 73: 370\u2013384.","journal-title":"FEMS Microbiol. Ecol."},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)75664-6"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200300599"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7799(00)01522-5"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0959-440X(03)00101-5"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002530100647"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/chir.530040513"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.6.2754-2762.2002"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(73)90342-4"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.12.5601-5607.2001"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01604-07"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.01.102"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol902280d"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200902835"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-011-3418-9"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2091-9-11"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.19.6200-6204.1999"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.64663-0"},{"key":"e_1_3_3_23_2","first-page":"3101","article-title":"Toluene and ethylbenzene oxidation by purified naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4","volume":"62","author":"Lee K","year":"1996","unstructured":"LeeK GibsonDT. 1996. Toluene and ethylbenzene oxidation by purified naphthalene dioxygenase from Pseudomonas sp. strain NCIB 9816-4. Appl. Environ. Mic robiol. 62: 3101\u20133106.","journal-title":"robiol."},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.46.100192.001425"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01899-06"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1099\/mic.0.2007\/014159-0"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/128_2006_072"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.63524-0"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0922-338X(92)90028-S"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.copbio.2004.06.011"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1994.1420"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00080-06"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/elsc.200700011"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00093-11"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0958-1669(02)00336-1"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06384-11"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(93)90613-8"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.2.796-804.2003"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.11.4216-4222.1997"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/chem.19970030406"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0141-0229(02)00129-1"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01434-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01434-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T19:30:50Z","timestamp":1645644650000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01434-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,9]]},"references-count":40,"journal-issue":{"issue":"17","published-print":{"date-parts":[[2012,9]]}},"alternative-id":["10.1128\/AEM.01434-12"],"URL":"https:\/\/doi.org\/10.1128\/aem.01434-12","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,9]]},"assertion":[{"value":"2012-05-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-06-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-08-09","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}