{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T10:58:30Z","timestamp":1790074710958,"version":"4.0.1"},"reference-count":71,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,10,1]],"date-time":"2020-10-01T00:00:00Z","timestamp":1601510400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Marie Curie Individual Fellowship","award":["H2020-MSCA-IF-2014"],"award-info":[{"award-number":["H2020-MSCA-IF-2014"]}]},{"DOI":"10.13039\/501100000268","name":"UKRI | Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/M011186\/1"],"award-info":[{"award-number":["BB\/M011186\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2020,10]]},"abstract":"<jats:p>\n                    Microbial symbionts of disease vectors have garnered recent attention due to their ability to alter vectorial capacity. Their consideration as a means of arbovirus control depends on symbiont vertical transmission, which leads to spread of the bacteria through a population. Previous work has identified a\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    symbiont present in several species of biting midges (\n                    <jats:italic>Culicoides<\/jats:italic>\n                    spp.), which transmit bluetongue and Schmallenberg arboviruses. However, symbiont transmission strategies and host effects remain underexplored. In this study, we describe the presence of\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    in the ovarian suspensory ligament of\n                    <jats:italic>Culicoides impunctatus<\/jats:italic>\n                    . Infection of this organ suggests the connective tissue surrounding developing eggs is important for ensuring vertical transmission of the symbiont in midges and possibly other insects. Additionally, our results indicate\n                    <jats:italic>Rickettsia<\/jats:italic>\n                    localization in the fat body of\n                    <jats:italic>Culicoides impunctatus<\/jats:italic>\n                    . As the arboviruses spread by midges often replicate in the fat body, this location implies possible symbiont-virus interactions to be further investigated.\n                  <\/jats:p>","DOI":"10.1128\/aem.01492-20","type":"journal-article","created":{"date-parts":[[2020,8,14]],"date-time":"2020-08-14T08:25:04Z","timestamp":1597393504000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":11,"title":["Tissue Tropisms and Transstadial Transmission of a\n                    <i>Rickettsia<\/i>\n                    Endosymbiont in the Highland Midge, Culicoides impunctatus (Diptera: Ceratopogonidae)"],"prefix":"10.1128","volume":"86","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2941-1482","authenticated-orcid":false,"given":"Jack","family":"Pilgrim","sequence":"first","affiliation":[{"name":"Institute of Infection, Veterinary and Ecological Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1104-7061","authenticated-orcid":false,"given":"Stefanos","family":"Siozios","sequence":"additional","affiliation":[{"name":"Institute of Infection, Veterinary and Ecological Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Baylis","sequence":"additional","affiliation":[{"name":"Institute of Infection, Veterinary and Ecological Sciences, Faculty of Health and Life Sciences, University of Liverpool, Liverpool, United Kingdom"},{"name":"Health Protection Research Unit in Emerging and Zoonotic Infections, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7163-7784","authenticated-orcid":false,"given":"Gregory D. 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