{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:55:26Z","timestamp":1790776526621,"version":"4.1.0"},"reference-count":53,"publisher":"American Society for Microbiology","license":[{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:00:00Z","timestamp":1790726400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32570031"],"award-info":[{"award-number":["32570031"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      <jats:italic toggle=\"yes\">Shewanella oneidensis<\/jats:italic>\n                      is one of the most extensively studied electrochemically active bacteria because of its unparalleled respiratory versatility, which is largely built on diverse cytochromes (cyts)\n                      <jats:italic toggle=\"yes\">c<\/jats:italic>\n                      . It has been firmly established that Fur, RyhB, and Hfq are critical for cyt\n                      <jats:italic toggle=\"yes\">c<\/jats:italic>\n                      production through regulation of iron homeostasis, but how they interplay remains largely unknown. In this study, we show that\n                      <jats:italic toggle=\"yes\">S. oneidensis<\/jats:italic>\n                      RyhB carries novel features, such as unusual length and functional exchangeability with its\n                      <jats:italic toggle=\"yes\">Escherichia coli<\/jats:italic>\n                      counterpart but not its\n                      <jats:italic toggle=\"yes\">Vibrio cholerae<\/jats:italic>\n                      counterpart. The RyhB loss suppresses the signature phenotypes caused by the Fur loss, including impaired growth and cyt\n                      <jats:italic toggle=\"yes\">c<\/jats:italic>\n                      production in\n                      <jats:italic toggle=\"yes\">S. oneidensis<\/jats:italic>\n                      . Despite this, neither the direct regulation of expression of most cyt\n                      <jats:italic toggle=\"yes\">c<\/jats:italic>\n                      genes nor the suppression of disrupted iron homeostasis resulting from Fur loss appears to be a primary mechanism underlying RyhB\u2019s influence on cyt\n                      <jats:italic toggle=\"yes\">c<\/jats:italic>\n                      content. Rather, this effect is achieved by modulating heme levels; RyhB mediates the expression of the key genes in heme biosynthesis and the entire heme uptake\/degradation system. Overall, our work highlights the significance of RyhB-mediated post-transcriptional regulation in the physiology of\n                      <jats:italic toggle=\"yes\">S. oneidensis<\/jats:italic>\n                      , unraveling unexpected mechanisms by which RyhB affects cyt\n                      <jats:italic toggle=\"yes\">c<\/jats:italic>\n                      biosynthesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      In bacteria, RyhB has been implicated in the regulation of diverse biological processes post-translationally. In\n                      <jats:italic toggle=\"yes\">Shewanella oneidensis<\/jats:italic>\n                      , both Fur and RyhB influence the cytochrome (cyt)\n                      <jats:italic toggle=\"yes\">c<\/jats:italic>\n                      content, which are the cornerstones of bacterial respiratory versatility and potential application in bioenergy and bioremediation. Here, we show that while RyhB generally exhibits an antagonistic relationship with Fur, it carries novel sequence features and differentially regulates heme biosynthesis and degradation, leading to altered cyt\n                      <jats:italic toggle=\"yes\">c<\/jats:italic>\n                      content. The data presented show that the biological processes in a given bacterium regulated by RyhB are highly complex, amounting to the required coordination of multiple regulators to allow cells to respond to environmental changes promptly.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01500-26","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:01:37Z","timestamp":1790773297000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Effects of RyhB on Fur regulation of cytochrome\n                    <i>c<\/i>\n                    synthesis in\n                    <i>Shewanella oneidensis<\/i>"],"prefix":"10.1128","author":[{"given":"Kaiyue","family":"Jie","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory for Vegetation Structure, Function and Construction (VegLab), Institute of Microbiology, College of Life Sciences, Zhejiang University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyue","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory for Vegetation Structure, Function and Construction (VegLab), Institute of Microbiology, College of Life Sciences, Zhejiang University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuqian","family":"Zhang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory for Vegetation Structure, Function and Construction (VegLab), Institute of Microbiology, College of Life Sciences, Zhejiang University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jim","family":"Yah","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory for Vegetation Structure, Function and Construction (VegLab), Institute of Microbiology, College of Life Sciences, Zhejiang University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7394-9821","authenticated-orcid":false,"given":"Lulu","family":"Liu","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory for Vegetation Structure, Function and Construction (VegLab), Institute of Microbiology, College of Life Sciences, Zhejiang University","place":["Hangzhou, China"]},{"id":[{"id":"https:\/\/ror.org\/052gg0110","id-type":"ROR","asserted-by":"publisher"}],"name":"Sir William Dunn School of Pathology, University of Oxford","place":["Oxford, United Kingdom"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2647-6563","authenticated-orcid":true,"given":"Haichun","family":"Gao","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/00a2xv884","id-type":"ROR","asserted-by":"publisher"}],"name":"State Key Laboratory for Vegetation Structure, Function and Construction (VegLab), Institute of Microbiology, College of Life Sciences, Zhejiang University","place":["Hangzhou, China"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","published-online":{"date-parts":[[2026,9,30]]},"reference":[{"key":"e_1_3_5_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro.2017.15"},{"key":"e_1_3_5_3_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV120.007746"},{"key":"e_1_3_5_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41580-023-00648-1"},{"key":"e_1_3_5_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.00022-23"},{"key":"e_1_3_5_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms5910"},{"key":"e_1_3_5_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1118321109"},{"key":"e_1_3_5_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/microbiolspec.rwr-0010-2017"},{"key":"e_1_3_5_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ymben.2025.02.002"},{"key":"e_1_3_5_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.01320-23"},{"key":"e_1_3_5_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/mlf2.12150"},{"key":"e_1_3_5_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1103185"},{"key":"e_1_3_5_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00039-18"},{"key":"e_1_3_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-024-06193-7"},{"key":"e_1_3_5_15_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.823758"},{"key":"e_1_3_5_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01230-25"},{"key":"e_1_3_5_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01752-18"},{"key":"e_1_3_5_18_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2020.593246"},{"key":"e_1_3_5_19_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2018.00268"},{"key":"e_1_3_5_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-13-33"},{"key":"e_1_3_5_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.01289-22"},{"key":"e_1_3_5_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.032066599"},{"key":"e_1_3_5_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.5706-5719.2005"},{"key":"e_1_3_5_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-10-264"},{"key":"e_1_3_5_25_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-11-129"},{"key":"e_1_3_5_26_2","doi-asserted-by":"publisher","DOI":"10.1186\/1748-7188-6-26"},{"key":"e_1_3_5_27_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_3_5_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.181.12.3792-3802.1999"},{"key":"e_1_3_5_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12865"},{"key":"e_1_3_5_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03034-3"},{"key":"e_1_3_5_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkx279"},{"key":"e_1_3_5_32_2","doi-asserted-by":"publisher","DOI":"10.1186\/s13059-023-03117-2"},{"key":"e_1_3_5_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/srep24449"},{"key":"e_1_3_5_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/mlf2.12120"},{"key":"e_1_3_5_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00203-16"},{"key":"e_1_3_5_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/10_2024_272"},{"key":"e_1_3_5_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA117.001530"},{"key":"e_1_3_5_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/mbio.03497-21"},{"key":"e_1_3_5_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.2.881-892.2002"},{"key":"e_1_3_5_40_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-9-S1-S11"},{"key":"e_1_3_5_41_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01154"},{"key":"e_1_3_5_42_2","doi-asserted-by":"publisher","DOI":"10.3390\/ijms232213841"},{"key":"e_1_3_5_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01700-19"},{"key":"e_1_3_5_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01989-19"},{"key":"e_1_3_5_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01255-18"},{"key":"e_1_3_5_46_2","doi-asserted-by":"publisher","DOI":"10.3390\/ncrna7040064"},{"key":"e_1_3_5_47_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00154-15"},{"key":"e_1_3_5_48_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0075610"},{"key":"e_1_3_5_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbalip.2018.06.020"},{"key":"e_1_3_5_50_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12457"},{"key":"e_1_3_5_51_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12091"},{"key":"e_1_3_5_52_2","volume-title":"A laboratory manual","author":"J Sambrook DR","year":"2016","unstructured":"J Sambrook DR. 2016. Molecular cloning: a laboratory manual. In A laboratory manual, 3rd ed. Molecular Cloning.","edition":"3"},{"key":"e_1_3_5_53_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00463-16"},{"key":"e_1_3_5_54_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00438-018-1487-5"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.01500-26","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/aem.01500-26","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T13:01:38Z","timestamp":1790773298000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/aem.01500-26"}},"subtitle":[],"editor":[{"given":"Julia C.","family":"van Kessel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2026,9,30]]},"references-count":53,"alternative-id":["10.1128\/aem.01500-26"],"URL":"https:\/\/doi.org\/10.1128\/aem.01500-26","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,30]]},"assertion":[{"value":"2026-08-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-01","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2026-09-30","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01500-26"}}