{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:14:39Z","timestamp":1790709279689,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"16","license":[{"start":{"date-parts":[[2015,8,15]],"date-time":"2015-08-15T00:00:00Z","timestamp":1439596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,8,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The aim of this study was to investigate the prevalence and transferability of resistance in tetracycline-resistant\n                    <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n                    isolates recovered from beef cattle in South Korea. A total of 155\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    isolates were collected from feces in South Korea, and 146 were confirmed to be resistant to tetracycline. The tetracycline resistance gene\n                    <jats:italic>tet<\/jats:italic>\n                    (A) (46.5%) was the most prevalent, followed by\n                    <jats:italic>tet<\/jats:italic>\n                    (B) (45.1%) and\n                    <jats:italic>tet<\/jats:italic>\n                    (C) (5.8%). Strains carrying\n                    <jats:italic>tet<\/jats:italic>\n                    (A) plus\n                    <jats:italic>tet<\/jats:italic>\n                    (B) and\n                    <jats:italic>tet<\/jats:italic>\n                    (B) plus\n                    <jats:italic>tet<\/jats:italic>\n                    (C) were detected in two isolates each. In terms of phylogenetic grouping, 101 (65.2%) isolates were classified as phylogenetic group B1, followed in decreasing order by D (17.4%), A (14.2%), and B2 (3.2%). Ninety-one (62.3%) isolates were determined to be multidrug resistant by the disk diffusion method. MIC testing using the principal tetracyclines, namely, tetracycline, chlortetracycline, oxytetracycline, doxycycline, and minocycline, revealed that isolates carrying\n                    <jats:italic>tet<\/jats:italic>\n                    (B) had higher MIC values than isolates carrying\n                    <jats:italic>tet<\/jats:italic>\n                    (A). Conjugation assays showed that 121 (82.9%) isolates could transfer a tetracycline resistance gene to a recipient via the IncFIB replicon (65.1%). This study suggests that the high prevalence of tetracycline-resistant\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    isolates in beef cattle is due to the transferability of tetracycline resistance genes between\n                    <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n                    populations which have survived the selective pressure caused by the use of antimicrobial agents.\n                  <\/jats:p>","DOI":"10.1128\/aem.01511-15","type":"journal-article","created":{"date-parts":[[2015,6,5]],"date-time":"2015-06-05T22:21:38Z","timestamp":1433542898000},"page":"5560-5566","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["Prevalence of Antimicrobial Resistance and Transfer of Tetracycline Resistance Genes in Escherichia coli Isolates from Beef Cattle"],"prefix":"10.1128","volume":"81","author":[{"given":"Seung Won","family":"Shin","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min Kyoung","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myunghwan","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuastros Mekonnen","family":"Belaynehe","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Han Sang","family":"Yoo","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases, College of Veterinary Medicine, Seoul National University, Seoul, Republic of Korea"},{"name":"Institute of Green Bio Science and Technology, Seoul National University, Pyeungchang, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0924-8579(02)00394-1"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0450.2004.00775.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1805.111153"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10156-010-0178-x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1145"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/MMBR.65.2.232-260.2001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.femsle.2005.02.034"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2011.01.003"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-006-0248-2"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02505-07"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(03)00123-8"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.50.1.156-161.2006"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00625-07"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12275-014-4166-1"},{"key":"e_1_3_2_16_2","volume-title":"M100-S23","author":"Clinical and Laboratory Standards Institute","year":"2013","unstructured":"Clinical and Laboratory Standards Institute. 2013. 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