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However, studies of large domestic animals, as well as challenge studies with pathogenic microorganisms, are very rare. Here, we investigated the influence of oral treatment of pigs with the probiotic bacterium<jats:italic>Enterococcus faecium<\/jats:italic>NCIMB 10415 on<jats:italic>Salmonella enterica<\/jats:italic>serovar Typhimurium DT104 infections in weaning piglets. Clinical symptoms, fecal excretion, the organ distribution of<jats:italic>Salmonella<\/jats:italic>, and the humoral immune response (immunoglobulin G [IgG], IgM, and IgA levels) in serum were examined. A pool of 89 piglets was randomly divided into probiotic and control groups. The probiotic group received a feed supplement containing<jats:italic>E. faecium<\/jats:italic>starting on day 14 postpartum prior to challenge with<jats:italic>Salmonella<\/jats:italic>serovar Typhimurium DT104 at 28 days postpartum. After challenge with<jats:italic>Salmonella<\/jats:italic>serovar Typhimurium DT104, piglets in both groups showed no severe clinical signs of salmonellosis. However, fecal excretion and colonization of<jats:italic>Salmonella<\/jats:italic>in organs were significantly greater in piglets fed<jats:italic>E. faecium<\/jats:italic>. Likewise, the humoral immune response against<jats:italic>Salmonella<\/jats:italic>(serum IgM and IgA levels) was significantly greater in the probiotic group animals than in control animals. The results of this study suggest that<jats:italic>E. faecium<\/jats:italic>NCIMB 10415 treatment enhanced the course of infection in weaning piglets challenged with<jats:italic>Salmonella<\/jats:italic>serovar Typhimurium DT104. However, the probiotic treatment also appeared to result in greater production of specific antibodies against<jats:italic>Salmonella<\/jats:italic>serovar Typhimurium DT104.<\/jats:p>","DOI":"10.1128\/aem.01515-08","type":"journal-article","created":{"date-parts":[[2009,3,7]],"date-time":"2009-03-07T02:10:18Z","timestamp":1236391818000},"page":"2621-2628","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":91,"title":["Influence of a Probiotic Strain of<i>Enterococcus faecium<\/i>on<i>Salmonella enterica<\/i>Serovar Typhimurium DT104 Infection in a Porcine Animal Infection Model"],"prefix":"10.1128","volume":"75","author":[{"given":"Istva\u0301n","family":"Szabo\u0301","sequence":"first","affiliation":[{"name":"Federal Institute for Risk Assessment, Division of Biological Safety, Diedersdorfer Weg 1, 12277 Berlin, Germany"},{"name":"Institute of Animal Hygiene and Environmental Health, Philippstrasse 13, 10115 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lothar H.","family":"Wieler","sequence":"additional","affiliation":[{"name":"Institute for Microbiology and Epizootics, Free University of Berlin, Philippstrasse 13, 10115 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"Tedin","sequence":"additional","affiliation":[{"name":"Institute for Microbiology and Epizootics, Free University of Berlin, Philippstrasse 13, 10115 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lydia","family":"Scharek-Tedin","sequence":"additional","affiliation":[{"name":"Institute of Immunology and Molecular Biology, Philippstrasse 13, 10115 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Taras","sequence":"additional","affiliation":[{"name":"Institute of Animal Nutrition, Free University of Berlin, Bru\u0308mmerstrasse 34, 14195 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreas","family":"Hensel","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment, Division of Biological Safety, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd","family":"Appel","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment, Division of Biological Safety, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karsten","family":"No\u0308ckler","sequence":"additional","affiliation":[{"name":"Federal Institute for Risk Assessment, Division of Biological Safety, Diedersdorfer Weg 1, 12277 Berlin, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"95","volume":"25","year":"1998","unstructured":"Baggesen, D. L., and F. M. Aarestrup. 1998. Characterisation of recently emerged multiple antibiotic-resistant Salmonella enterica serovar Typhimurium DT 104 and other multiresistant phage types from Danish pig herds. Vet. Rec.25:95-97.","journal-title":"Vet. Rec."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1079\/PNS2001118"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/133.4.1158"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(96)00990-7"},{"key":"e_1_3_2_6_2","first-page":"71","year":"1997","unstructured":"Blaha, T., G. Solano-Aguillar, and C. Pijoan. 1997. The early colonization pattern of Salmonella Typhimurium in pigs after oral intake, p. 71-73. 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