{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T23:40:09Z","timestamp":1791589209639,"version":"4.3.4"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2017,11,1]],"date-time":"2017-11-01T00:00:00Z","timestamp":1509494400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004564","name":"Ministarstvo Prosvete, Nauke i Tehnolo\u0161kog Razvoja","doi-asserted-by":"publisher","award":["172049"],"award-info":[{"award-number":["172049"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004564","name":"Ministarstvo Prosvete, Nauke i Tehnolo\u0161kog Razvoja","doi-asserted-by":"publisher","award":["173019"],"award-info":[{"award-number":["173019"]}],"id":[{"id":"10.13039\/501100004564","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Lactococcus lactis<\/jats:named-content>\n                    subsp.\n                    <jats:italic>lactis<\/jats:italic>\n                    bv. diacetylactis BGBU1-4 produces a novel bacteriocin, lactolisterin BU, with strong antimicrobial activity against many species of Gram-positive bacteria, including important food spoilage and foodborne pathogens, such as\n                    <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Bacillus<\/jats:named-content>\n                    spp., and streptococci. Lactolisterin BU was extracted from the cell surface of BGBU1-4 by 2-propanol and purified to homogeneity by C\n                    <jats:sub>18<\/jats:sub>\n                    solid-phase extraction and reversed-phase high-performance liquid chromatography. The molecular mass of the purified lactolisterin BU was 5,160.94 Da, and an internal fragment, AVSWAWQH, as determined by N-terminal sequencing, showed low-level similarity to existing antimicrobial peptides. Curing and transformation experiments revealed the presence of a corresponding bacteriocin operon on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4. Analysis of the bacteriocin operon revealed a leaderless bacteriocin of 43 amino acids that exhibited similarity to bacteriocin BHT-B (63%) from\n                    <jats:named-content content-type=\"genus-species\">Streptococcus ratti<\/jats:named-content>\n                    , a bacteriocin with analogy to aureocin A.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:bold>IMPORTANCE<\/jats:bold>\n                    Lactolisterin BU, a broad-spectrum leaderless bacteriocin produced by\n                    <jats:named-content content-type=\"genus-species\">L. lactis<\/jats:named-content>\n                    subsp.\n                    <jats:italic>lactis<\/jats:italic>\n                    bv. diacetylactis BGBU1-4, expresses strong antimicrobial activity against food spoilage and foodborne pathogens, such as\n                    <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Bacillus<\/jats:named-content>\n                    spp., and streptococci. Lactolisterin BU showed the highest similarity to aureocin-like bacteriocins produced by different bacteria. The operon for synthesis is located on the smallest plasmid, pBU6 (6.2 kb), of strain BGBU1-4, indicating possible horizontal transfer among producers.\n                  <\/jats:p>","DOI":"10.1128\/aem.01519-17","type":"journal-article","created":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T20:25:41Z","timestamp":1503692741000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":36,"title":["Lactolisterin BU, a Novel Class II Broad-Spectrum Bacteriocin from Lactococcus lactis subsp.\n                    <i>lactis<\/i>\n                    bv. diacetylactis BGBU1-4"],"prefix":"10.1128","volume":"83","author":[{"given":"Jelena","family":"Lozo","sequence":"first","affiliation":[{"name":"Faculty of Biology, University of Belgrade, Belgrade, Serbia"},{"name":"Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nemanja","family":"Mirkovic","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paula M.","family":"O'Connor","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Centre Cork, Moorepark, Fermoy, County Cork, Ireland"},{"name":"APC Microbiome Institute, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milka","family":"Malesevic","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marija","family":"Miljkovic","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Natalija","family":"Polovic","sequence":"additional","affiliation":[{"name":"Faculty of Chemistry, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9500-3786","authenticated-orcid":false,"given":"Branko","family":"Jovcic","sequence":"additional","affiliation":[{"name":"Faculty of Biology, University of Belgrade, Belgrade, Serbia"},{"name":"Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul D.","family":"Cotter","sequence":"additional","affiliation":[{"name":"Teagasc Food Research Centre Cork, Moorepark, Fermoy, County Cork, Ireland"},{"name":"APC Microbiome Institute, Cork, Ireland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Milan","family":"Kojic","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics and Genetic Engineering, University of Belgrade, Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2007.00683.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11274-009-9984-7"},{"key":"e_1_3_3_4_2","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1007\/978-1-4419-7692-5_1","volume-title":"Prokaryotic antimicrobial peptides\u2014from genes to applications","author":"Nes IF","year":"2011","unstructured":"Nes IF . 2011. 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