{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T03:34:39Z","timestamp":1791171279741,"version":"4.1.0"},"reference-count":64,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Autotransporters are secreted bacterial proteins exhibiting diverse virulence functions. Various autotransporters have been identified among<jats:italic>Escherichia coli<\/jats:italic>associated with intestinal or extraintestinal infections; however, the specific distribution of autotransporter sequences among a diversity of<jats:italic>E. coli<\/jats:italic>strains has not been investigated. We have validated the use of a multiplex PCR assay to screen for the presence of autotransporter sequences. Herein, we determined the presence of 13 autotransporter sequences and five allelic variants of antigen 43 (Ag43) among 491<jats:italic>E. coli<\/jats:italic>isolates from human urinary tract infections, diarrheagenic<jats:italic>E. coli<\/jats:italic>, and avian pathogenic<jats:italic>E. coli<\/jats:italic>(APEC) and<jats:italic>E. coli<\/jats:italic>reference strains belonging to the ECOR collection. Clinical isolates were also classified into established phylogenetic groups. The results indicated that Ag43 alleles were significantly associated with clinical isolates (93%) compared to commensal isolates (56%) and that<jats:italic>agn43K12<\/jats:italic>was the most common and widely distributed allele.<jats:italic>agn43<\/jats:italic>allelic variants were also phylogenetically distributed. Sequences encoding<jats:italic>espC<\/jats:italic>,<jats:italic>espP<\/jats:italic>, and<jats:italic>sepA<\/jats:italic>and<jats:italic>agn43<\/jats:italic>alleles<jats:italic>EDL933<\/jats:italic>and<jats:italic>RS218<\/jats:italic>were significantly associated with diarrheagenic<jats:italic>E. coli<\/jats:italic>strains compared to other groups.<jats:italic>tsh<\/jats:italic>was highly associated with APEC strains, whereas<jats:italic>sat<\/jats:italic>was absent from APEC.<jats:italic>vat<\/jats:italic>,<jats:italic>sat<\/jats:italic>, and<jats:italic>pic<\/jats:italic>were associated with urinary tract isolates and were identified predominantly in isolates belonging to either group B2 or D of the phylogenetic groups based on the ECOR strain collection. Overall, the results indicate that specific autotransporter sequences are associated with the source and\/or phylogenetic background of strains and suggest that, in some cases, autotransporter gene profiles may be useful for comparative analysis of<jats:italic>E. coli<\/jats:italic>strains from clinical, food, and environmental sources.<\/jats:p>","DOI":"10.1128\/aem.01542-06","type":"journal-article","created":{"date-parts":[[2007,1,13]],"date-time":"2007-01-13T01:35:27Z","timestamp":1168652127000},"page":"1553-1562","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":120,"title":["Autotransporter-Encoding Sequences Are Phylogenetically Distributed among<i>Escherichia coli<\/i>Clinical Isolates and Reference Strains"],"prefix":"10.1128","volume":"73","author":[{"given":"Concetta","family":"Restieri","sequence":"first","affiliation":[{"name":"INRS-Institut Armand-Frappier, Laval, Que\u0301bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genevie\u0300ve","family":"Garriss","sequence":"additional","affiliation":[{"name":"INRS-Institut Armand-Frappier, Laval, Que\u0301bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Claude","family":"Locas","sequence":"additional","affiliation":[{"name":"INRS-Institut Armand-Frappier, Laval, Que\u0301bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles M.","family":"Dozois","sequence":"additional","affiliation":[{"name":"INRS-Institut Armand-Frappier, Laval, Que\u0301bec, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Ausubel F. 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