{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T07:36:48Z","timestamp":1791185808520,"version":"4.1.0"},"reference-count":76,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2014,10,15]],"date-time":"2014-10-15T00:00:00Z","timestamp":1413331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Members of the enterobacterial genus\n            <jats:named-content content-type=\"genus-species\">Serratia<\/jats:named-content>\n            are ecologically widespread, and some strains are opportunistic human pathogens. Bacteriophage \u03d5MAM1 was isolated on\n            <jats:named-content content-type=\"genus-species\">Serratia plymuthica<\/jats:named-content>\n            A153, a biocontrol rhizosphere strain that produces the potently bioactive antifungal and anticancer haterumalide oocydin A. The \u03d5MAM1 phage is a generalized transducing phage that infects multiple environmental and clinical isolates of\n            <jats:named-content content-type=\"genus-species\">Serratia<\/jats:named-content>\n            spp. and a rhizosphere strain of\n            <jats:named-content content-type=\"genus-species\">Kluyvera cryocrescens<\/jats:named-content>\n            . Electron microscopy allowed classification of \u03d5MAM1 in the family\n            <jats:named-content content-type=\"genus-species\">Myoviridae<\/jats:named-content>\n            . Bacteriophage \u03d5MAM1 is virulent, uses capsular polysaccharides as a receptor, and can transduce chromosomal markers at frequencies of up to 7 \u00d7 10\n            <jats:sup>\u22126<\/jats:sup>\n            transductants per PFU. We also demonstrated transduction of the complete 77-kb oocydin A gene cluster and heterogeneric transduction of a plasmid carrying a type III toxin-antitoxin system. These results support the notion of the potential ecological importance of transducing phages in the acquisition of genes by horizontal gene transfer. Phylogenetic analyses grouped \u03d5MAM1 within the ViI-like bacteriophages, and genomic analyses revealed that the major differences between \u03d5MAM1 and other ViI-like phages arise in a region encoding the host recognition determinants. Our results predict that the wider genus of ViI-like phages could be efficient transducing phages, and this possibility has obvious implications for the ecology of horizontal gene transfer, bacterial functional genomics, and synthetic biology.\n          <\/jats:p>","DOI":"10.1128\/aem.01546-14","type":"journal-article","created":{"date-parts":[[2014,8,9]],"date-time":"2014-08-09T04:55:20Z","timestamp":1407560120000},"page":"6446-6457","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Bacteriophage \u03d5MAM1, a Viunalikevirus, Is a Broad-Host-Range, High-Efficiency Generalized Transducer That Infects Environmental and Clinical Isolates of the Enterobacterial Genera Serratia and Kluyvera"],"prefix":"10.1128","volume":"80","author":[{"given":"Miguel A.","family":"Matilla","sequence":"first","affiliation":[{"name":"Department of Biochemistry, University of Cambridge, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George P. 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