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In this study, we investigated the ability of the extremophilic yeast\n                      <jats:italic toggle=\"yes\">Debaryomyces hansenii<\/jats:italic>\n                      to activate specialized detoxification mechanisms for BaP degradation, even under nutrient-deprived conditions. When exposed to 100 ppm BaP,\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      eliminated over 70% of the contaminant within 3 days, while maintaining normal growth dynamics. RNA-Seq analysis revealed widespread transcriptional remodeling, with 1,179 genes upregulated and 1,031 downregulated under BaP-only conditions, and 1,067 upregulated and 977 downregulated genes during cometabolic exposure (2% glucose + 100 ppm BaP), from a total of 6,506 annotated genes. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses highlighted the activation of xenobiotic degradation pathways, notably involving cytochrome P450 monooxygenases, epoxide hydrolases, and glutathione S-transferases, alongside an enhanced antioxidant response and finely tuned glutathione homeostasis. This work presents the transcriptomic profile of BaP detoxification in\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      , which reveals a complex transcriptional activation of genes related to stress adaptation and detoxification. Collectively, these findings suggest that\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      could represent a promising eukaryotic platform for bioremediation.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Polycyclic aromatic hydrocarbons (PAHs), such as benzo(a)pyrene (BaP), are long-lasting environmental pollutants with serious health and ecological implications. Although microbial degradation offers a promising strategy for remediation, most efforts have focused on bacterial and filamentous fungal systems, leaving other microbial groups comparatively unexplored. In contrast, extremotolerant yeasts remain largely overlooked despite their inherent resilience. Here, we investigated the marine yeast\n                      <jats:italic toggle=\"yes\">Debaryomyces hansenii<\/jats:italic>\n                      and discovered that it not only tolerates BaP under glucose-limited conditions but also actively degrades it. This response relies on a combination of detoxifying enzymes and antioxidant defenses, reflecting a well-orchestrated metabolic adaptation to chemical stress. Our findings underscore the untapped and promising potential of\n                      <jats:italic toggle=\"yes\">D. hansenii<\/jats:italic>\n                      as a robust option for bioremediation using eukaryotic organisms, particularly in environments where conventional microbes may fail to survive.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01557-25","type":"journal-article","created":{"date-parts":[[2025,9,16]],"date-time":"2025-09-16T13:01:03Z","timestamp":1758027663000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":4,"title":["Transcriptomic profiling of\n                    <i>Debaryomyces hansenii<\/i>\n                    reveals detoxification and stress responses to benzo(a)pyrene 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