{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T13:59:57Z","timestamp":1790171997848,"version":"4.1.0"},"reference-count":55,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T00:00:00Z","timestamp":1790121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2026,9,23]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title\/>\n                    <jats:p>\n                      Truffle fungi, such as\n                      <jats:italic toggle=\"yes\">Tuber melanosporum<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">T. aestivum<\/jats:italic>\n                      , are prized luxury food products that have been historically cultivated in Mediterranean regions of Europe. Recent advances in truffle cultivation have led to the establishment of productive truffle orchards in North America, South America, China, and Australia. Despite these advances, a consistent issue has been the introduction and accidental cultivation of non-target truffle species. Non-target truffle species (e.g.,\n                      <jats:italic toggle=\"yes\">T. indicum<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">T. brumale<\/jats:italic>\n                      ) often morphologically resemble target species (e.g.,\n                      <jats:italic toggle=\"yes\">T. melanosporum<\/jats:italic>\n                      ) and may unintentionally be included as an inoculum and introduced to seedlings. Detecting inoculum and seedling contamination is difficult and often relies on inconsistent morphological analyses or labor-intensive and time-consuming PCR-based methods. Furthermore, quantitative PCR (qPCR) assays have only been developed for\n                      <jats:italic toggle=\"yes\">T. melanosporum<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">T. aestivum,<\/jats:italic>\n                      leaving truffle growers without rapid tools to detect important contaminants such as\n                      <jats:italic toggle=\"yes\">T. brumale<\/jats:italic>\n                      . Here, we develop a rapid loop-mediated isothermal amplification (LAMP) assay to detect three globally cultivated\n                      <jats:italic toggle=\"yes\">Tuber<\/jats:italic>\n                      species (\n                      <jats:italic toggle=\"yes\">T. melanosporum<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">T. borchii<\/jats:italic>\n                      , and\n                      <jats:italic toggle=\"yes\">T. aestivum<\/jats:italic>\n                      ) and the most common introduced contaminant species (\n                      <jats:italic toggle=\"yes\">T. brumale<\/jats:italic>\n                      ). The assays presented here are sensitive to at least 10\n                      <jats:sup>\u22123<\/jats:sup>\n                      ng of DNA and are species-specific. These assays should be of special interest to both truffle orchard owners and truffle testing facilities given the low time commitment, high sensitivity, high specificity, and cost comparable to PCR and qPCR-based approaches.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>IMPORTANCE<\/jats:title>\n                    <jats:p>\n                      Despite the global success of truffle cultivation, the accidental cultivation of undesirable truffle species has been a persistent issue. To address this issue, we developed four loop-mediated isothermal amplification (LAMP) assays for the rapid detection of\n                      <jats:italic toggle=\"yes\">Tuber aestivum<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">T. borchii<\/jats:italic>\n                      ,\n                      <jats:italic toggle=\"yes\">T. brumale,<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">T. melanosporum<\/jats:italic>\n                      from ectomycorrhizal root tips and truffle fruiting bodies. These assays are alternatives to the widely used\n                      <jats:italic toggle=\"yes\">T. aestivum<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">T. melanosporum<\/jats:italic>\n                      qPCR assays and are the first rapid detection assays for\n                      <jats:italic toggle=\"yes\">T. borchii<\/jats:italic>\n                      and\n                      <jats:italic toggle=\"yes\">T. brumale<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01559-26","type":"journal-article","created":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:00:38Z","timestamp":1787922038000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["Rapid detection of cultivated truffle fungi (\n                    <i>Tuber<\/i>\n                    ) from truffles and ectomycorrhizal root tips using loop-mediated isothermal amplification"],"prefix":"10.1128","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7240-2207","authenticated-orcid":true,"given":"Alassane","family":"Sow","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02y3ad647","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant Pathology, University of Florida","place":["Gainesville, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-1430-2687","authenticated-orcid":true,"given":"Andrew","family":"Cooper","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04tj63d06","id-type":"ROR","asserted-by":"publisher"}],"name":"Mountain Research Station, North Carolina State University","place":["Waynesville, 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