{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T12:26:28Z","timestamp":1791462388472,"version":"4.3.4"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2020,1,21]],"date-time":"2020-01-21T00:00:00Z","timestamp":1579564800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004543","name":"Chinese Scholarship council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2020,1,21]]},"abstract":"<jats:p>\n            <jats:italic>Penicillium rubens<\/jats:italic>\n            is an important industrial producer of \u03b2-lactam antibiotics. High levels of penicillin production were enforced through extensive mutagenesis during a classical strain improvement (CSI) program over 70 years. Several mutations targeted amino acid metabolism and resulted in enhanced\n            <jats:sc>l<\/jats:sc>\n            -cysteine biosynthesis. This work provides a molecular explanation for the interrelation between secondary metabolite production and amino acid metabolism and how classical strain improvement has resulted in improved production strains.\n          <\/jats:p>","DOI":"10.1128\/aem.01561-19","type":"journal-article","created":{"date-parts":[[2019,11,21]],"date-time":"2019-11-21T11:06:32Z","timestamp":1574334392000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Impact of Classical Strain Improvement of\n            <i>Penicillium rubens<\/i>\n            on Amino Acid Metabolism during \u03b2-Lactam Production"],"prefix":"10.1128","volume":"86","author":[{"given":"Min","family":"Wu","sequence":"first","affiliation":[{"name":"Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciprian G.","family":"Crismaru","sequence":"additional","affiliation":[{"name":"Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oleksandr","family":"Salo","sequence":"additional","affiliation":[{"name":"Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roel A. 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