{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T05:46:31Z","timestamp":1769147191209,"version":"3.49.0"},"reference-count":48,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2011,2,15]],"date-time":"2011-02-15T00:00:00Z","timestamp":1297728000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,2,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>Internalin A (InlA; encoded by<jats:italic>inlA<\/jats:italic>) facilitates the crossing of the intestinal barrier by<jats:italic>Listeria monocytogenes<\/jats:italic>. Mutations leading to a premature stop codon (PMSC) in<jats:italic>inlA<\/jats:italic>and thus attenuated mammalian virulence have been reported. We recently characterized 502<jats:italic>L. monocytogenes<\/jats:italic>food isolates from a retail survey and 507 human clinical isolates from multiple U.S. states with respect to the presence\/absence of<jats:italic>inlA<\/jats:italic>mutations. The objective of this study was to investigate the hypothesis that dose responses for human listeriosis vary between<jats:italic>L. monocytogenes<\/jats:italic>strains with and those without a PMSC in<jats:italic>inlA<\/jats:italic>. Subtype-specific prevalence and concentration distributions in food, along with epidemiologic and consumption data, were input into established dose-response models to generate an<jats:italic>r<\/jats:italic>value (probability of a cell causing illness). Under the conservative assumption that<jats:italic>L. monocytogenes<\/jats:italic>levels at retail represent levels consumed, mean log<jats:sub>10<\/jats:sub><jats:italic>r<\/jats:italic>values were \u22128.1 and \u221210.7 for<jats:italic>L. monocytogenes<\/jats:italic>subtypes with genes encoding a full-length and a truncated InlA, respectively.<jats:italic>L. monocytogenes<\/jats:italic>carrying a 5\u2032 frameshift mutation in a homopolymeric tract showed a mean log<jats:sub>10<\/jats:sub><jats:italic>r<\/jats:italic>value of \u221212.1. Confidence intervals for the<jats:italic>r<\/jats:italic>values and their differences varied depending on subtypes. When the increase in concentration of<jats:italic>L. monocytogenes<\/jats:italic>subtypes between retail and consumption was considered, mean log<jats:sub>10<\/jats:sub><jats:italic>r<\/jats:italic>values were reduced to \u221210.4, \u221213.8, and \u221212.8 for the subtypes with genes encoding a full-length InlA, for the subtypes carrying a PMSC in<jats:italic>inlA<\/jats:italic>, and for all<jats:italic>L. monocytogenes<\/jats:italic>isolates regardless of subtype, respectively. Our study provides further quantitative evidence that<jats:italic>L. monocytogenes<\/jats:italic>subtypes vary in abilities and relative likelihoods of causing human disease, which were mechanistically related to defined genetic markers.<\/jats:p>","DOI":"10.1128\/aem.01564-10","type":"journal-article","created":{"date-parts":[[2010,12,18]],"date-time":"2010-12-18T04:06:21Z","timestamp":1292645181000},"page":"1171-1180","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Variation in<i>Listeria monocytogenes<\/i>Dose Responses in Relation to Subtypes Encoding a Full-Length or Truncated Internalin A"],"prefix":"10.1128","volume":"77","author":[{"given":"Yuhuan","family":"Chen","sequence":"first","affiliation":[{"name":"Grocery Manufacturers Association, Washington, D.C"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William H.","family":"Ross","sequence":"additional","affiliation":[{"name":"Food Directorate, Health Canada, Ottawa, Ontario, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard C.","family":"Whiting","sequence":"additional","affiliation":[{"name":"Exponent, Bowie, Maryland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anna","family":"Van Stelten","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Colorado State University, Fort Collins, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kendra K.","family":"Nightingale","sequence":"additional","affiliation":[{"name":"Department of Animal Science, Colorado State University, Fort Collins, Colorado"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martin","family":"Wiedmann","sequence":"additional","affiliation":[{"name":"Department of Food Science, Cornell University, Ithaca, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Virginia N.","family":"Scott","sequence":"additional","affiliation":[{"name":"Grocery Manufacturers Association, Washington, D.C"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.1.489-497.2004"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-60.8.918"},{"key":"e_1_3_2_4_2","first-page":"530","volume":"7","year":"2007","unstructured":"Cates, S. 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