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Huanglongbing (HLB) is a serious disease of citrus for which there is currently no cure. Here, we tested the hypothesis that bacteria native to the citrus microbiome may possess inhibitory activity against the associated causal agent of HLB,\n                      <jats:italic toggle=\"yes\">Candidatus<\/jats:italic>\n                      Liberibacter asiaticus (\n                      <jats:italic toggle=\"yes\">C<\/jats:italic>\n                      Las). We coupled a robotic microfluidic isolation platform with a high-throughput antimicrobial screening assay using\n                      <jats:italic toggle=\"yes\">Liberibacter crescens<\/jats:italic>\n                      , a culturable surrogate for the unculturable\n                      <jats:italic toggle=\"yes\">C<\/jats:italic>\n                      Las, to identify isolates with anti-\n                      <jats:italic toggle=\"yes\">Liberibacter<\/jats:italic>\n                      activity. We identified 35 bacterial isolates with activity using this pipeline. One strongly inhibitory citrus root isolate,\n                      <jats:italic toggle=\"yes\">Pseudomonas chlororaphis<\/jats:italic>\n                      (SRAM11), was selected for bioassay-guided fractionation. We determined a significant driver of\n                      <jats:italic toggle=\"yes\">in vitro Liberibacter<\/jats:italic>\n                      inhibition was attributed to the production of phenazine-1-carboxamide (PCN) by\n                      <jats:italic toggle=\"yes\">P. chlororaphis<\/jats:italic>\n                      . We also demonstrated significant anti-\n                      <jats:italic toggle=\"yes\">C<\/jats:italic>\n                      Las activity of PCN, comparable to that of oxytetracycline, using an\n                      <jats:italic toggle=\"yes\">ex vivo<\/jats:italic>\n                      citrus hairy root assay.\n                      <jats:italic toggle=\"yes\">P. chlororaphis<\/jats:italic>\n                      and phenazines have been implicated in successful biocontrol of other diseases and represent a new tool with possible application to HLB mitigation.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Huanglongbing (HLB) is a devastating citrus disease that causes millions of dollars in crop loss each year in the United States alone. In this study, we mined the citrus microbiome for bacteria that produce antimicrobials with suppressive activity against\n                        <jats:italic toggle=\"yes\">Candidatus<\/jats:italic>\n                        Liberibacter asiaticus, the causal agent of HLB. We demonstrated that the citrus microbiome is a rich source of microorganisms that inhibit\n                        <jats:italic toggle=\"yes\">Liberibacter<\/jats:italic>\n                        spp. We selected a\n                        <jats:italic toggle=\"yes\">Pseudomonas chlororaphis<\/jats:italic>\n                        strain for further characterization and assigned anti-\n                        <jats:italic toggle=\"yes\">Liberibacter<\/jats:italic>\n                        activity to the production of phenazine-1-carboxamide. Phenazine-producing\n                        <jats:italic toggle=\"yes\">P. chlororaphis<\/jats:italic>\n                        strains are known biocontrols in other pathosystems, and this study highlights their potential for mitigation of HLB.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01576-26","type":"journal-article","created":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T13:00:27Z","timestamp":1791896427000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":0,"title":["A high-throughput pipeline for isolation and screening of the citrus bacteriome identifies\n                    <i>Pseudomonas chlororaphis<\/i>\n                    with anti-\n                    <i>Candidatus<\/i>\n                    Liberibacter asiaticus activity"],"prefix":"10.1128","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5306-3257","authenticated-orcid":true,"given":"Christopher","family":"Drozd","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03nawhv43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Plant Pathology, University of California, Riverside","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn","family":"Maebori","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03nawhv43","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Plant Pathology, University of California, Riverside","place":["Riverside, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolina","family":"Ruiz Rivera","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kiana","family":"Callahan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1788-241X","authenticated-orcid":true,"given":"Manikandan","family":"Ramasamy","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant Pathology and Microbiology, Texas A&M University","place":["College Station, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kranthi","family":"Mandadi","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Plant Pathology and Microbiology, Texas A&M University","place":["College Station, USA"]},{"id":[{"id":"https:\/\/ror.org\/01f5ytq51","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute for Advancing Health Through Agriculture, Texas A&M AgriLife","place":["College Station, USA"]},{"name":"Texas A&M AgriLife Research & Extension Center, Texas A&M University System","place":["Weslaco, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3472-7593","authenticated-orcid":true,"given":"Katherine N.","family":"Maloney","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02rsjqy82","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Chemistry, Point Loma Nazarene University","place":["San Diego, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4637-6225","authenticated-orcid":true,"given":"M. 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