{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T13:54:07Z","timestamp":1790258047822,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2019,10,1]],"date-time":"2019-10-01T00:00:00Z","timestamp":1569888000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001804","name":"Canada Research Chairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001804","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:p>\n            <jats:italic>Bacillus<\/jats:italic>\n            spp. are considered pressure-resistant microorganisms, but the resistance mechanisms remain unknown. The\n            <jats:italic>spoVA<\/jats:italic>\n            <jats:sup>2mob<\/jats:sup>\n            operon is a mobile genetic element, and it can transfer to pathogenic or spoilage organisms by horizontal gene transfer. Results in this study indicate that multiple copies of the\n            <jats:italic>spoVA<\/jats:italic>\n            <jats:sup>2mob<\/jats:sup>\n            operon mediate high-pressure resistance of\n            <jats:italic>Bacillus<\/jats:italic>\n            endospores, and it might contribute to the identification of the source of pressure-resistant pathogens and spoilage organisms that may contaminate the food supply. The droplet digital PCR (ddPCR) system is well suited for analysis in some human diseases due to its high efficiency and capability to provide high precision; however, no relevant studies in food microbiology have been reported so far. This study demonstrates a novel application of ddPCR in food microbiology.\n          <\/jats:p>","DOI":"10.1128\/aem.01596-19","type":"journal-article","created":{"date-parts":[[2019,8,2]],"date-time":"2019-08-02T11:38:32Z","timestamp":1564745912000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["The Copy Number of the\n            <i>spoVA<\/i>\n            <sup>2mob<\/sup>\n            Operon Determines Pressure Resistance of\n            <i>Bacillus<\/i>\n            Endospores"],"prefix":"10.1128","volume":"85","author":[{"given":"Zhen","family":"Li","sequence":"first","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Felix","family":"Schottroff","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada"},{"name":"Vienna Institute of Food Technology, University of Natural Resources and Life Sciences (BOKU), Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5757-641X","authenticated-orcid":false,"given":"David J.","family":"Simpson","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0972-928X","authenticated-orcid":false,"given":"Michael G.","family":"G\u00e4nzle","sequence":"additional","affiliation":[{"name":"Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mib.2003.10.001"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2016.01912"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-24904-9_22"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tifs.2003.08.005"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3234-4_2"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2013.03.001"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1541-4337.2007.00021.x"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.5.3476-3481.2006"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-67.11.2530"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-food-030117-012808"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.12.7321-7328.2004"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01469-18"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/ismej.2016.59"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01483-18"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03122-16"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.13386"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2012.12.010"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.66.7.2829-2834.2000"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.03755-12"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2007.02204.x"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01613-06"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/mmi.12591"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12859-017-1705-x"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1229164"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1152\/physiolgenomics.00082.2012"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2014.08.004"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1021\/ac202578x"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2633"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02620-12"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0062583"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0055943"},{"key":"e_1_3_3_33_2","first-page":"30","article-title":"Fadenziehende bakterien-vorkommen, bedeutung gegenma\u00dfnahmen","volume":"47","author":"R\u00f6cken W","year":"1993","unstructured":"R\u00f6cken W, Spicher G. 1993. 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