{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:18:39Z","timestamp":1791303519480,"version":"4.1.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2008,3,1]],"date-time":"2008-03-01T00:00:00Z","timestamp":1204329600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2008,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Two phylogenetic methods (multilocus sequence typing [MLST] and a multiplex PCR) were investigated to determine whether phylogenetic classification of verocytotoxin-producing\n            <jats:italic>Escherichia coli<\/jats:italic>\n            serotypes correlates with their classification into groups (seropathotypes A to E) based on their relative incidence in human disease and on their association with outbreaks and serious complications. MLST was able to separate 96% of seropathotype D and E serotypes from those that cause serious disease (seropathotypes A to C), whereas the multiplex PCR lacked this level of seropathotype discrimination.\n          <\/jats:p>","DOI":"10.1128\/aem.01619-07","type":"journal-article","created":{"date-parts":[[2007,12,29]],"date-time":"2007-12-29T01:49:17Z","timestamp":1198892957000},"page":"1671-1675","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Applicability of Phylogenetic Methods for Characterizing the Public Health Significance of Verocytotoxin-Producing\n            <i>Escherichia coli<\/i>\n            Strains"],"prefix":"10.1128","volume":"74","author":[{"given":"Kim","family":"Ziebell","sequence":"first","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Konczy","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Yong","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shelley","family":"Frost","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariola","family":"Mascarenhas","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Kropinski","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"Whittam","sequence":"additional","affiliation":[{"name":"Microbial Evolution Laboratory, National Food Safety and Toxicology Center, Michigan State University, East Lansing, Michigan 48824"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan C.","family":"Read","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Karmali","sequence":"additional","affiliation":[{"name":"Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, Guelph, Ontario N1G 3W4, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.10.4555-4558.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI12404"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msh118"},{"key":"e_1_3_2_5_2","unstructured":"Edward and Ewing's identification of Enterobacteriaceae 1986"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.12.6098-6107.2005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.172.11.6175-6181.1990"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.4930-4940.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.18.3.775-779.1977"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/5.2.150"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(00)00223-0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.157.2.690-693.1984"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.2.598-602.1998"},{"key":"e_1_3_2_14_2","unstructured":"Molecular cloning: a laboratory manual 2001"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.46157-0"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_3_2_17_2","first-page":"195","year":"1998","unstructured":"Whittam, T. 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