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This selective activity is achieved through targeting of the mannose phosphotransferase system (manPTS) of a subset of Gram-positive bacteria, although factors affecting this mechanism are not entirely understood. Peptides identified from genomic data, as well as variants of previously characterized AMPs, can offer insight into how peptide sequence affects activity and selectivity. The experimental methods presented here identify promising potent and selective bacteriocins for further evaluation, highlight the potential of simple computational modeling for prediction of AMP performance, and demonstrate that factors beyond manPTS sequence affect bacterial susceptibility to class IIa bacteriocins.<\/jats:p>","DOI":"10.1128\/aem.01646-20","type":"journal-article","created":{"date-parts":[[2020,9,14]],"date-time":"2020-09-14T14:32:04Z","timestamp":1600093924000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":10,"title":["Mining and Statistical Modeling of Natural and Variant Class IIa Bacteriocins Elucidate Activity and Selectivity Profiles across Species"],"prefix":"10.1128","volume":"86","author":[{"given":"Daniel T.","family":"Tresnak","sequence":"first","affiliation":[{"name":"Department of Chemical Engineering and Materials Science, University of Minnesota\u2014Twin Cities, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3561-9463","authenticated-orcid":false,"given":"Benjamin J.","family":"Hackel","sequence":"additional","affiliation":[{"name":"Department of Chemical Engineering and Materials Science, University of Minnesota\u2014Twin Cities, Minneapolis, Minnesota, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","volume-title":"Antibiotic resistance threats in the United States, 2013","author":"Centers for Disease Control and Prevention","year":"2013","unstructured":"Centers for Disease Control and Prevention. 2013. 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