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Consequently, data on environmental\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      and other\n                      <jats:italic>Acinetobacter<\/jats:italic>\n                      species remain limited. Here, we utilized Illumina and Nanopore sequencing to analyze the genomes of 10\n                      <jats:italic>Acinetobacter<\/jats:italic>\n                      isolates representing 6 different species sourced from aquatic environments in South Australia. All 10 isolates were phylogenetically distinct compared to clinical and other non-clinical\n                      <jats:italic>Acinetobacter<\/jats:italic>\n                      strains, often tens of thousands of single-nucleotide polymorphisms from their nearest neighbors. Despite the genetic divergence, we identified p\n                      <jats:italic>dif<\/jats:italic>\n                      modules (sections of mobilized DNA) carrying clinically important antimicrobial resistance genes in species other than\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      , including carbapenemase\n                      <jats:italic>oxa58,<\/jats:italic>\n                      tetracycline resistance gene\n                      <jats:italic>tet(39<\/jats:italic>\n                      ), and macrolide resistance genes\n                      <jats:italic>msr(E)-mph(E<\/jats:italic>\n                      ). These p\n                      <jats:italic>dif<\/jats:italic>\n                      modules were located on plasmids with high sequence identity to those circulating in globally distributed\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      ST1 and ST2 clones. The environmental\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      isolate characterized here (SAAb472; ST350) did not possess any native plasmids; however, it could capture two clinically important plasmids (pRAY and pACICU2) with high transfer frequencies. Furthermore,\n                      <jats:italic>A. baumannii<\/jats:italic>\n                      SAAb472 possessed virulence genes and a capsular polysaccharide type analogous to clinical strains. Our findings highlight the potential for environmental\n                      <jats:italic>Acinetobacter<\/jats:italic>\n                      species to acquire and disseminate clinically important antimicrobial resistance genes, underscoring the need for further research into the ecology and evolution of this important genus.\n                    <\/jats:p>\n                    <jats:sec>\n                      <jats:title>IMPORTANCE<\/jats:title>\n                      <jats:p>\n                        Antimicrobial resistance (AMR) is a global threat to human, animal, and environmental health. Studying AMR in environmental bacteria is crucial to understand the emergence and dissemination of resistance genes and pathogens, and to identify potential reservoirs and transmission routes. This study provides novel insights into the genomic diversity and AMR potential of environmental\n                        <jats:italic>Acinetobacter<\/jats:italic>\n                        species. By comparing the genomes of aquatic\n                        <jats:italic>Acinetobacter<\/jats:italic>\n                        isolates with clinical and non-clinical strains, we revealed that they are highly divergent yet carry p\n                        <jats:italic>dif<\/jats:italic>\n                        modules that encode resistance to antibiotics commonly used in clinical settings. We also demonstrated that an environmental\n                        <jats:italic>A. baumannii<\/jats:italic>\n                        isolate can acquire clinically relevant plasmids and carries virulence factors similar to those of hospital-associated strains. These findings suggest that environmental\n                        <jats:italic>Acinetobacter<\/jats:italic>\n                        species may serve as reservoirs and vectors of clinically important genes. Consequently, further research is warranted to comprehensively understand the ecology and evolution of this genus.\n                      <\/jats:p>\n                    <\/jats:sec>\n                  <\/jats:sec>","DOI":"10.1128\/aem.01654-23","type":"journal-article","created":{"date-parts":[[2024,1,11]],"date-time":"2024-01-11T09:00:33Z","timestamp":1704963633000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Genomic analysis of diverse environmental\n                    <i>Acinetobacter<\/i>\n                    isolates identifies plasmids, antibiotic resistance genes, and capsular polysaccharides shared with clinical strains"],"prefix":"10.1128","volume":"90","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2840-4790","authenticated-orcid":false,"given":"Liam A.","family":"Tobin","sequence":"first","affiliation":[{"name":"Australian Institute for Microbiology and Infection, University of 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Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3301-0470","authenticated-orcid":false,"given":"Barbara","family":"Drigo","sequence":"additional","affiliation":[{"name":"Future Industries Institute, University of South Australia, Mawson Lakes, SA, Australia"},{"name":"UniSA STEM, University of South Australia, Mawson Lakes, SA, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erica","family":"Donner","sequence":"additional","affiliation":[{"name":"Future Industries Institute, University of South Australia, Mawson Lakes, SA, Australia"},{"name":"Cooperative Research Centre for Solving Antimicrobial Resistance in Agribusiness, Food, and Environments (CRC SAAFE), Adelaide, SA, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9301-5372","authenticated-orcid":false,"given":"Steven 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