{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,29]],"date-time":"2026-06-29T19:07:05Z","timestamp":1782760025450,"version":"3.54.5"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2019,11,15]],"date-time":"2019-11-15T00:00:00Z","timestamp":1573776000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"JSPS KAKENHI","award":["JP18K08428"],"award-info":[{"award-number":["JP18K08428"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2019,11,15]]},"abstract":"<jats:p>\n            Colistin is considered a last-line therapeutic option in severe infections due to multidrug-resistant Gram-negative bacteria, in particular carbapenemase-producing\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            and multidrug-resistant\n            <jats:named-content content-type=\"genus-species\">Acinetobacter baumannii<\/jats:named-content>\n            . An increasing prevalence of\n            <jats:italic>mcr<\/jats:italic>\n            genes in diverse\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            species, mainly\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            from humans and food animals, has become a significant concern to public health all over the world. In Japan,\n            <jats:italic>mcr<\/jats:italic>\n            genes have so far been detected in food animals, raw meat, wastewater, and human clinical samples. This study reports the copresence of\n            <jats:italic>mcr-1<\/jats:italic>\n            and avian pathogenic\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            (APEC)-associated virulence genes in five of seven\n            <jats:named-content content-type=\"genus-species\">E. coli<\/jats:named-content>\n            isolates recovered from aquatic environments in Japan. Our study highlights the importance and urgency of action to reduce environmental contamination by\n            <jats:italic>mcr<\/jats:italic>\n            genes that may likely occur due to exposure to untreated wastewater through combined sewer overflow by recent unusual weather.\n          <\/jats:p>","DOI":"10.1128\/aem.01661-19","type":"journal-article","created":{"date-parts":[[2019,9,6]],"date-time":"2019-09-06T11:51:52Z","timestamp":1567770712000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Acquisition of\n            <i>mcr-1<\/i>\n            and Cocarriage of Virulence Genes in Avian Pathogenic Escherichia coli Isolates from Municipal Wastewater Influents in Japan"],"prefix":"10.1128","volume":"85","author":[{"given":"Wataru","family":"Hayashi","sequence":"first","affiliation":[{"name":"Department of Medical Sciences, Shinshu University Graduate School of Medicine, Science and Technology, 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