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The<jats:italic>in vivo<\/jats:italic>protein target has been reported for all seven serotypes of BoNT, i.e., serotypes A to G. Knowledge of the cleavage sites has led to the development of several assays to detect BoNT based on its ability to cleave a peptide substrate derived from its<jats:italic>in vivo<\/jats:italic>protein target. Most serotypes of BoNT can be subdivided into subtypes, and previously, we demonstrated that three of the currently known subtypes of BoNT\/F cleave a peptide substrate, a shortened version of synaptobrevin-2, between Q58 and K59. However, our research indicated that<jats:italic>Clostridium baratii<\/jats:italic>type F toxin did not cleave this peptide. In this study, we detail experiments demonstrating that<jats:italic>Clostridium baratii<\/jats:italic>type F toxin cleaves recombinant synaptobrevin-2 in the same location as that cleaved by proteolytic F toxin. In addition, we demonstrate that<jats:italic>Clostridium baratii<\/jats:italic>type F toxin can cleave a peptide substrate based on the sequence of synaptobrevin-2. This peptide substrate is an N-terminal extension of the original peptide substrate used for detection of other BoNT\/F toxins and can be used to detect four of the currently known BoNT\/F subtypes by mass spectrometry.<\/jats:p>","DOI":"10.1128\/aem.01662-10","type":"journal-article","created":{"date-parts":[[2010,12,18]],"date-time":"2010-12-18T04:06:21Z","timestamp":1292645181000},"page":"1301-1308","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":21,"title":["Different Substrate Recognition Requirements for Cleavage of Synaptobrevin-2 by<i>Clostridium baratii<\/i>and<i>Clostridium botulinum<\/i>Type F Neurotoxins"],"prefix":"10.1128","volume":"77","author":[{"given":"Suzanne R.","family":"Kalb","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy. 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Detrick, Maryland 21702"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leonard A.","family":"Smith","sequence":"additional","affiliation":[{"name":"Integrated Toxicology, United States Army Medical Research Institute of Infectious Diseases (USAMRIID), Ft. Detrick, Maryland 21702"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James L.","family":"Pirkle","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy. N.E., Atlanta, Georgia 30341"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Barr","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, National Center for Environmental Health, Division of Laboratory Sciences, 4770 Buford Hwy. 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