{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:40:17Z","timestamp":1790815217091,"version":"4.1.0"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2014,10,15]],"date-time":"2014-10-15T00:00:00Z","timestamp":1413331200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2014,10,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The mechanisms and rates of mercury methylation in the Florida Everglades are of great concern because of potential adverse impacts on human and wildlife health through mercury accumulation in aquatic food webs. We developed a new PCR primer set targeting\n                    <jats:italic>hgcA<\/jats:italic>\n                    , a gene encoding a corrinoid protein essential for Hg methylation across broad phylogenetic boundaries, and used this primer set to study the distribution of\n                    <jats:italic>hgcA<\/jats:italic>\n                    sequences in soils collected from three sites along a gradient in sulfate and nutrient concentrations in the northern Everglades. The sequences obtained were distributed in diverse phyla, including\n                    <jats:named-content content-type=\"genus-species\">Proteobacteria<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Chloroflexi<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Firmicutes<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">Methanomicrobia<\/jats:named-content>\n                    ; however,\n                    <jats:italic>hgcA<\/jats:italic>\n                    clone libraries from all sites were dominated by sequences clustering within the order\n                    <jats:named-content content-type=\"genus-species\">Syntrophobacterales<\/jats:named-content>\n                    of the\n                    <jats:named-content content-type=\"genus-species\">Deltaproteobacteria<\/jats:named-content>\n                    (49 to 65% of total sequences).\n                    <jats:italic>dsrB<\/jats:italic>\n                    mRNA sequences, representing active sulfate-reducing prokaryotes at the time of sampling, obtained from these sites were also dominated by\n                    <jats:named-content content-type=\"genus-species\">Syntrophobacterales<\/jats:named-content>\n                    (75 to 89%). Laboratory incubations with soils taken from the site low in sulfate concentrations also suggested that Hg methylation activities were primarily mediated by members of the order\n                    <jats:named-content content-type=\"genus-species\">Syntrophobacterales<\/jats:named-content>\n                    , with some contribution by methanogens,\n                    <jats:named-content content-type=\"genus-species\">Chloroflexi<\/jats:named-content>\n                    , iron-reducing\n                    <jats:named-content content-type=\"genus-species\">Geobacter<\/jats:named-content>\n                    , and non-sulfate-reducing\n                    <jats:named-content content-type=\"genus-species\">Firmicutes<\/jats:named-content>\n                    inhabiting the sites. This suggests that prokaryotes distributed within clades defined by syntrophs are the predominant group controlling methylation of Hg in low-sulfate areas of the Everglades. Any strategy for managing mercury methylation in the Everglades should consider that net mercury methylation is not limited to the action of sulfate reduction.\n                  <\/jats:p>","DOI":"10.1128\/aem.01666-14","type":"journal-article","created":{"date-parts":[[2014,8,9]],"date-time":"2014-08-09T00:55:20Z","timestamp":1407545720000},"page":"6517-6526","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":101,"title":["Syntrophs Dominate Sequences Associated with the Mercury Methylation-Related Gene\n                    <i>hgcA<\/i>\n                    in the Water Conservation Areas of the Florida Everglades"],"prefix":"10.1128","volume":"80","author":[{"given":"Hee-Sung","family":"Bae","sequence":"first","affiliation":[{"name":"Soil and Water Science Department, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Forrest E.","family":"Dierberg","sequence":"additional","affiliation":[{"name":"DB Environmental Inc., Rockledge, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Ogram","sequence":"additional","affiliation":[{"name":"Soil and Water Science Department, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"3A-1","volume-title":"2014 South Florida environmental report","author":"Julian P","year":"2014","unstructured":"JulianPIIPayneGGXueSK. 2014. 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