{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T13:59:44Z","timestamp":1769522384371,"version":"3.49.0"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"19","license":[{"start":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T00:00:00Z","timestamp":1380585600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Many methanotrophs have been shown to synthesize methanobactin, a novel biogenic copper-chelating agent or chalkophore. Methanobactin binds copper via two heterocyclic rings with associated enethiol groups. The structure of methanobactin suggests that it can bind other metals, including mercury. Here we report that methanobactin from\n            <jats:named-content content-type=\"genus-species\">Methylosinus trichosporium<\/jats:named-content>\n            OB3b does indeed bind mercury when added as HgCl\n            <jats:sub>2<\/jats:sub>\n            and, in doing so, reduced toxicity associated with Hg(II) for both\n            <jats:named-content content-type=\"genus-species\">Alphaproteobacteria<\/jats:named-content>\n            methanotrophs, including\n            <jats:named-content content-type=\"genus-species\">M. trichosporium<\/jats:named-content>\n            OB3b,\n            <jats:named-content content-type=\"genus-species\">M. trichosporium<\/jats:named-content>\n            OB3b \u0394\n            <jats:italic>mbnA<\/jats:italic>\n            (a mutant defective in methanobactin production), and\n            <jats:named-content content-type=\"genus-species\">Methylocystis<\/jats:named-content>\n            sp. strain SB2, and a\n            <jats:named-content content-type=\"genus-species\">Gammaproteobacteria<\/jats:named-content>\n            methanotroph,\n            <jats:named-content content-type=\"genus-species\">Methylomicrobium album<\/jats:named-content>\n            BG8. Mercury binding by methanobactin was evident in both the presence and absence of copper, despite the fact that methanobactin had a much higher affinity for copper due to the rapid and irreversible binding of mercury by methanobactin. The formation of a gray precipitate suggested that Hg(II), after being bound by methanobactin, was reduced to Hg(0) but was not volatilized. Rather, mercury remained associated with methanobactin and was also found associated with methanotrophic biomass. It thus appears that although the mercury-methanobactin complex was cell associated, mercury was not removed from methanobactin. The amount of biomass-associated mercury in the presence of methanobactin from\n            <jats:named-content content-type=\"genus-species\">M. trichosporium<\/jats:named-content>\n            OB3b was greatest for\n            <jats:named-content content-type=\"genus-species\">M. trichosporium<\/jats:named-content>\n            wild-type strain OB3b and the \u0394\n            <jats:italic>mbnA<\/jats:italic>\n            mutant and least for\n            <jats:named-content content-type=\"genus-species\">M. album<\/jats:named-content>\n            BG8, suggesting that methanotrophs may have selective methanobactin uptake systems that may be based on TonB-dependent transporters but that such uptake systems exhibit a degree of infidelity.\n          <\/jats:p>","DOI":"10.1128\/aem.01673-13","type":"journal-article","created":{"date-parts":[[2013,7,20]],"date-time":"2013-07-20T05:08:50Z","timestamp":1374296930000},"page":"5918-5926","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Detoxification of Mercury by Methanobactin from Methylosinus trichosporium OB3b"],"prefix":"10.1128","volume":"79","author":[{"given":"Alexey","family":"Vorobev","sequence":"first","affiliation":[{"name":"Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheeja","family":"Jagadevan","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bipin S.","family":"Baral","sequence":"additional","affiliation":[{"name":"Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan A.","family":"DiSpirito","sequence":"additional","affiliation":[{"name":"Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brittani C.","family":"Freemeier","sequence":"additional","affiliation":[{"name":"Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandt H.","family":"Bergman","sequence":"additional","affiliation":[{"name":"Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nathan L.","family":"Bandow","sequence":"additional","affiliation":[{"name":"Roy J. Carver Department of Biochemistry, Biophysics and Molecular Biology, Iowa State University, Ames, Iowa, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeremy D.","family":"Semrau","sequence":"additional","affiliation":[{"name":"Department of Civil and Environmental Engineering, The University of Michigan, Ann Arbor, Michigan, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6976.2010.00212.x"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00132233"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.3.1106-1114.1998"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.19.5755-5764.2003"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/1462-2920.12150"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/0162-0134(94)00056-G"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.178.4.1018-1029.1996"},{"key":"e_1_3_3_10_2","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/978-1-4020-3163-2_7","volume-title":"Respiration in archaea and bacteria","author":"DiSpirito AA","year":"2004","unstructured":"DiSpiritoAAKunzRCChoiDWZahnJA. 2004. 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