{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T20:56:32Z","timestamp":1791406592156,"version":"4.3.3"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T00:00:00Z","timestamp":1173916800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The diversity and dynamics of bacterial populations in Saint-Nectaire, a raw-milk, semihard cheese, were investigated using a dual culture-dependent and direct molecular approach combining single-strand conformation polymorphism (SSCP) fingerprinting and sequencing of 16S rRNA genes. The dominant clones, among 125 16S rRNA genes isolated from milk, belonged to members of the\n            <jats:italic>Firmicutes<\/jats:italic>\n            (58% of the total clones) affiliated mainly with the orders\n            <jats:italic>Clostridiales<\/jats:italic>\n            and the\n            <jats:italic>Lactobacillales<\/jats:italic>\n            , followed by the phyla\n            <jats:italic>Proteobacteria<\/jats:italic>\n            (21.6%),\n            <jats:italic>Actinobacteria<\/jats:italic>\n            (16.8%), and\n            <jats:italic>Bacteroidetes<\/jats:italic>\n            (4%). Sequencing the 16S rRNA genes of 126 milk isolates collected from four culture media revealed the presence of 36 different species showing a wider diversity in the\n            <jats:italic>Gammaproteobacteria<\/jats:italic>\n            phylum and\n            <jats:italic>Staphylococcus<\/jats:italic>\n            genus than that found among clones. In cheese, a total of 21 species were obtained from 170 isolates, with dominant species belonging to the\n            <jats:italic>Lactobacillales<\/jats:italic>\n            and subdominant species affiliated with the\n            <jats:italic>Actinobacteria<\/jats:italic>\n            ,\n            <jats:italic>Bacteroidetes<\/jats:italic>\n            (\n            <jats:italic>Chryseobacterium<\/jats:italic>\n            sp.), or\n            <jats:italic>Gammaproteobacteria<\/jats:italic>\n            (\n            <jats:italic>Stenotrophomonas<\/jats:italic>\n            sp.). Fingerprinting DNA isolated from milk by SSCP analysis yielded complex patterns, whereas analyzing DNA isolated from cheese resulted in patterns composed of a single peak which corresponded to that of lactic acid bacteria. SSCP fingerprinting of mixtures of all colonies harvested from plate count agar supplemented with crystal violet and vancomycin showed good potential for monitoring the subdominant\n            <jats:italic>Proteobacteria<\/jats:italic>\n            and\n            <jats:italic>Bacteroidetes<\/jats:italic>\n            (\n            <jats:italic>Flavobacteria<\/jats:italic>\n            ) organisms in milk and cheese. Likewise, analyzing culturable subcommunities from cheese-ripening bacterial medium permitted assessment of the diversity of halotolerant\n            <jats:italic>Actinobacteria<\/jats:italic>\n            and\n            <jats:italic>Staphylococcus<\/jats:italic>\n            organisms. Direct and culture-dependent approaches produced complementary information, thus generating a more accurate view of milk and cheese microbial ecology.\n          <\/jats:p>","DOI":"10.1128\/aem.01716-06","type":"journal-article","created":{"date-parts":[[2007,1,27]],"date-time":"2007-01-27T01:34:07Z","timestamp":1169861647000},"page":"1882-1891","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":199,"title":["Monitoring Bacterial Communities in Raw Milk and Cheese by Culture-Dependent and -Independent 16S rRNA Gene-Based Analyses"],"prefix":"10.1128","volume":"73","author":[{"given":"Ce\u0301line","family":"Delbe\u0300s","sequence":"first","affiliation":[{"name":"INRA, UR545, Unite\u0301 de Recherches Fromage\u0300res, 36 rue de Salers, 15000 Aurillac, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leila","family":"Ali-Mandjee","sequence":"additional","affiliation":[{"name":"INRA, UR545, 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