{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T16:18:40Z","timestamp":1791303520090,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2007,3,1]],"date-time":"2007-03-01T00:00:00Z","timestamp":1172707200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Feedlot cattle were observed for fecal excretion of and rectoanal junction (RAJ) colonization with\n            <jats:italic>Escherichia coli<\/jats:italic>\n            O157:H7 to identify potential \u201csupershedders.\u201d RAJ colonization and fecal excretion prevalences were correlated, and\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 prevalences and counts were significantly greater for RAJ samples. Based on a comparison of RAJ and fecal ratios of\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7\/\n            <jats:italic>E. coli<\/jats:italic>\n            counts, the RAJ appears to be preferentially colonized by the O157:H7 serotype. Five supershedders were identified based on persistent colonization with high concentrations of\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7. Cattle copenned with supershedders had significantly greater mean pen\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 RAJ and fecal prevalences than noncopenned cattle. Cumulative fecal\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 excretion was also significantly higher for pens housing a supershedder.\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7\/\n            <jats:italic>E. coli<\/jats:italic>\n            count ratios were higher for supershedders than for other cattle, indicating greater proportional colonization. Pulsed-field gel electrophoresis analysis demonstrated that isolates from supershedders and copenned cattle were highly related. Cattle that remained negative for\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 throughout sampling were five times more likely to have been in a pen that did not house a supershedder. The data from this study support an association between levels of fecal excretion of\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 and RAJ colonization in pens of feedlot cattle and suggest that the presence of supershedders influences group-level excretion parameters. An improved understanding of individual and population transmission dynamics of\n            <jats:italic>E. coli<\/jats:italic>\n            O157:H7 can be used to develop preslaughter- and slaughter-level interventions that reduce contamination of the food chain.\n          <\/jats:p>","DOI":"10.1128\/aem.01742-06","type":"journal-article","created":{"date-parts":[[2007,1,13]],"date-time":"2007-01-13T01:35:27Z","timestamp":1168652127000},"page":"1563-1568","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":148,"title":["Rectoanal Junction Colonization of Feedlot Cattle by\n            <i>Escherichia coli<\/i>\n            O157:H7 and Its Association with Supershedders and Excretion Dynamics"],"prefix":"10.1128","volume":"73","author":[{"given":"Rowland N.","family":"Cobbold","sequence":"first","affiliation":[{"name":"Field Disease Investigation Unit, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-6610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dale D.","family":"Hancock","sequence":"additional","affiliation":[{"name":"Field Disease Investigation Unit, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-6610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel H.","family":"Rice","sequence":"additional","affiliation":[{"name":"Field Disease Investigation Unit, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-6610"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janice","family":"Berg","sequence":"additional","affiliation":[{"name":"Lakeside Research, Brooks, Alberta T1R 1B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert","family":"Stilborn","sequence":"additional","affiliation":[{"name":"Lakeside Research, Brooks, Alberta T1R 1B7, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn J.","family":"Hovde","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Molecular Biology, and Biochemistry, University of Idaho, Moscow, Idaho 83844-3052"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas E.","family":"Besser","sequence":"additional","affiliation":[{"name":"Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-7040"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a017914"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2004.02465.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-67.4.666"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S095026880100615X"},{"key":"e_1_3_2_6_2","first-page":"57","volume":"50","year":"1997","unstructured":"Buzby, J. C., and T. Roberts. 1997. Economic costs and trade impacts of microbial foodborne illness. World Health Stat. Q.50:57-66.","journal-title":"World Health Stat. Q."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5877(03)00050-3"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268803001432"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.5.3766-3770.2006"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.3.451"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.3.656-662.1997"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-68.9.1926"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.10.6431-6433.2005"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00487-6"},{"key":"e_1_3_2_15_2","first-page":"85","year":"1998","unstructured":"Hancock, D. D., T. E. Besser, and D. H. Rice. 1998. Ecology of Escherichia coli O157:H7 in cattle and impact of management practices, p. 85-91. In J. B. Kaper and A. D. O'Brien (ed.), Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains. ASM Press, Washington, DC.","journal-title":"Escherichia coli O157:H7 and other Shiga toxin-producing E. coli strains"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2005.06.009"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-66.11.1972"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.1.93-97.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268805004590"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1079\/AHR200463"},{"key":"e_1_3_2_21_2","unstructured":"National Cattlemen's Beef Association. E. coli O157:H7 solutions: the farm to table continuum. Presented at the Beef Industry E. coli Summit Meeting San Antonio TX 7 to 8 January 2003."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.71.3.1505-1512.2003"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2004.tb09495.x"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0890-8508(05)80014-3"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.69.5.2444-2447.2003"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.4924-4929.2003"},{"key":"e_1_3_2_27_2","unstructured":"World Health Organization. 2005. Enterohaemorrhagic Escherichia coli (EHEC). http:\/\/www.who.int\/mediacentre\/factsheets\/fs125\/en\/\n. Accessed 7 June 2006."}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01742-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01742-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T16:24:13Z","timestamp":1645633453000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01742-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3]]},"references-count":26,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,3]]}},"alternative-id":["10.1128\/AEM.01742-06"],"URL":"https:\/\/doi.org\/10.1128\/aem.01742-06","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3]]},"assertion":[{"value":"2006-07-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-12-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}