{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T17:49:20Z","timestamp":1792000160160,"version":"4.4.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2015,9,15]],"date-time":"2015-09-15T00:00:00Z","timestamp":1442275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2015,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The objective of this study was to evaluate the progression of the uterine microbiota from calving until establishment of metritis. Uterine swabs (\n                    <jats:italic>n<\/jats:italic>\n                    = 72) collected at 0, 2, and 6 \u00b1 2 days postpartum (dpp) from 12 metritic and 12 healthy cows were used for metagenomic sequencing of the 16S rRNA gene on the Illumina MiSeq platform. A heat map showed that uterine microbiota was established at calving. The microbiota changed rapidly from 0 to 6 \u00b1 2 dpp, with a decrease in the abundance of\n                    <jats:named-content content-type=\"genus-species\">Proteobacteria<\/jats:named-content>\n                    and an increase in the abundance of\n                    <jats:named-content content-type=\"genus-species\">Bacteroidetes<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">Fusobacteria<\/jats:named-content>\n                    , which were dominant in metritic cows. Uterine microbiota composition was shared; however, metritic and healthy cows could be discriminated using relative abundance of bacterial genera at 0, 2, and 6 \u00b1 2 dpp.\n                    <jats:named-content content-type=\"genus-species\">Bacteroides<\/jats:named-content>\n                    was the main genus associated with metritis because it was the only genus that showed significantly greater abundance in cows with metritis. As the abundance of\n                    <jats:named-content content-type=\"genus-species\">Bacteroides<\/jats:named-content>\n                    organisms increased, the uterine discharge score, a measure of uterine health, worsened.\n                    <jats:named-content content-type=\"genus-species\">Fusobacterium<\/jats:named-content>\n                    was also an important genus associated with metritis because\n                    <jats:named-content content-type=\"genus-species\">Fusobacterium<\/jats:named-content>\n                    abundance increased as\n                    <jats:named-content content-type=\"genus-species\">Bacteroides<\/jats:named-content>\n                    abundance increased and the uterine discharge score worsened as the abundance increased. The correlation with uterine discharge score and the correlation with\n                    <jats:named-content content-type=\"genus-species\">Bacteroides<\/jats:named-content>\n                    or\n                    <jats:named-content content-type=\"genus-species\">Fusobacterium<\/jats:named-content>\n                    showed that other bacteria, such as\n                    <jats:named-content content-type=\"genus-species\">Helcoccocus<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Filifactor<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">Porphyromonas<\/jats:named-content>\n                    , were also associated with metritis. There were also bacteria associated with uterine health, such as \u201c\n                    <jats:named-content content-type=\"genus-species\">Candidatus<\/jats:named-content>\n                    Blochmannia,\u201d\n                    <jats:named-content content-type=\"genus-species\">Escherichia<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Sneathia<\/jats:named-content>\n                    , and\n                    <jats:named-content content-type=\"genus-species\">Pedobacter<\/jats:named-content>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/aem.01753-15","type":"journal-article","created":{"date-parts":[[2015,7,6]],"date-time":"2015-07-06T22:50:27Z","timestamp":1436223027000},"page":"6324-6332","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":162,"title":["Uterine Microbiota Progression from Calving until Establishment of Metritis in Dairy Cows"],"prefix":"10.1128","volume":"81","author":[{"given":"Soo Jin","family":"Jeon","sequence":"first","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Achilles","family":"Vieira-Neto","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohanathas","family":"Gobikrushanth","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo","family":"Daetz","sequence":"additional","affiliation":[{"name":"Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodolfo D.","family":"Mingoti","sequence":"additional","affiliation":[{"name":"Department of Animal Sciences, University of Florida, Gainesville, Florida, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Carolina Brigolin","family":"Parize","sequence":"additional","affiliation":[{"name":"Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s, Goi\u00e2nia, Goi\u00e1s, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sabrina Lucas","family":"de Freitas","sequence":"additional","affiliation":[{"name":"Escola de Veterin\u00e1ria e Zootecnia, Universidade Federal de Goi\u00e1s, Goi\u00e2nia, Goi\u00e1s, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio Nelson Lima","family":"da Costa","sequence":"additional","affiliation":[{"name":"Departamento de Zootecnia, Universidade Federal do Cear\u00e1, Fortaleza, Cear\u00e1, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo C.","family":"Bicalho","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Svetlana","family":"Lima","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, Cornell University, Ithaca, New York, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"K. 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