{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T03:07:37Z","timestamp":1784603257240,"version":"3.55.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"21","license":[{"start":{"date-parts":[[2019,11,1]],"date-time":"2019-11-01T00:00:00Z","timestamp":1572566400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000060","name":"HHS | NIH | National Institute of Allergy and Infectious Diseases","doi-asserted-by":"publisher","award":["U01-AI124316"],"award-info":[{"award-number":["U01-AI124316"]}],"id":[{"id":"10.13039\/100000060","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:p>\n            As researchers try to understand and combat the development of antibiotic resistance in pathogens, there is a growing need to thoroughly understand the physiology and metabolism of the microbes.\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            is a threatening pathogen with increased antibiotic resistance and well-studied virulence mechanisms. However, the adaptive mechanisms used by this pathogen to survive environmental stresses remain unclear, mostly due to the lack of information about its metabolic requirements. Defining the minimal metabolic requirements for\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            growth is a first step toward unraveling the mechanisms by which it adapts to metabolic stresses. Here, we present the development of a chemically defined minimal medium supporting growth of three\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            strains, and we reveal key genetic mutations contributing to improved growth in minimal medium.\n          <\/jats:p>","DOI":"10.1128\/aem.01773-19","type":"journal-article","created":{"date-parts":[[2019,8,29]],"date-time":"2019-08-29T09:47:48Z","timestamp":1567072068000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Strain-Specific Metabolic Requirements Revealed by a Defined Minimal Medium for Systems Analyses of\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"85","author":[{"given":"Henrique","family":"Machado","sequence":"first","affiliation":[{"name":"Department of Bioengineering, University of California San Diego, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liam L.","family":"Weng","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California San Diego, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas","family":"Dillon","sequence":"additional","affiliation":[{"name":"Collaborative to Halt Antibiotic-Resistant Microbes, Department of Pediatrics, University of California San Diego, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yara","family":"Seif","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California San Diego, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle","family":"Holland","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California San Diego, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan E.","family":"Pekar","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California San Diego, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan M.","family":"Monk","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California San Diego, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3847-0422","authenticated-orcid":false,"given":"Victor","family":"Nizet","sequence":"additional","affiliation":[{"name":"Collaborative to Halt Antibiotic-Resistant Microbes, Department of Pediatrics, University of California San Diego, La Jolla, California, USA"},{"name":"Skaggs School of Pharmacy and Pharmaceutical Sciences, University of California San Diego, La Jolla, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard O.","family":"Palsson","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California San Diego, La Jolla, California, USA"},{"name":"Collaborative to Halt Antibiotic-Resistant Microbes, Department of Pediatrics, University of California San Diego, La Jolla, California, USA"},{"name":"Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam M.","family":"Feist","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, University of California San Diego, La Jolla, California, USA"},{"name":"Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00134-14"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.12.4665-4672.2004"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.2014.859985"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1307797110"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1401"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki372"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2015.05.021"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ebiom.2017.05.026"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1021\/acsinfecdis.7b00194"},{"key":"e_1_3_3_11_2","first-page":"322","article-title":"The nutrition of Staphylococcus aureus: nitrogen requirements","volume":"18","author":"Gladstone GP","year":"1937","unstructured":"Gladstone GP. 1937. The nutrition of Staphylococcus aureus: nitrogen requirements. Br J Exp Pathol 18:322\u2013333.","journal-title":"Br J Exp Pathol"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj0310731"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-34-3-143"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/am.15.4.866-870.1967"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/am.25.5.800-806.1973"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.118.1.155-164.1974"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01743-08"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-013-1100-7"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01434-16"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.3365"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-017-8627-4"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1932"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg3564"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02246-14"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/femspd\/ftx005"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.00220-10"},{"key":"e_1_3_3_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01545-07"},{"key":"e_1_3_3_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01492-08"},{"key":"e_1_3_3_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.06582-11"},{"key":"e_1_3_3_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.00537-12"},{"key":"e_1_3_3_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3589-3593.2004"},{"key":"e_1_3_3_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/msv033"},{"key":"e_1_3_3_33_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-018-0340-2"},{"key":"e_1_3_3_34_2","doi-asserted-by":"publisher","DOI":"10.1139\/m75-107"},{"key":"e_1_3_3_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiotec.2014.01.023"},{"key":"e_1_3_3_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/pro.2262"},{"key":"e_1_3_3_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.01514-08"},{"key":"e_1_3_3_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.6.1843-1852.2001"},{"key":"e_1_3_3_39_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002030050428"},{"key":"e_1_3_3_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.23.6824-6826.2000"},{"key":"e_1_3_3_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00026-16"},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.01255-08"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.02253-16"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.15252\/msb.20145949"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbs086"},{"key":"e_1_3_3_46_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1005595"},{"key":"e_1_3_3_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu153"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01773-19","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01773-19","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T19:01:14Z","timestamp":1645642874000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01773-19"}},"subtitle":[],"editor":[{"given":"Charles M.","family":"Dozois","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2019,11]]},"references-count":46,"journal-issue":{"issue":"21","published-print":{"date-parts":[[2019,11]]}},"alternative-id":["10.1128\/AEM.01773-19"],"URL":"https:\/\/doi.org\/10.1128\/aem.01773-19","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,11]]},"assertion":[{"value":"2019-08-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-08-26","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2019-10-16","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e01773-19"}}