{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T17:51:13Z","timestamp":1789581073357,"version":"build-2803163510"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"22","license":[{"start":{"date-parts":[[2018,11,15]],"date-time":"2018-11-15T00:00:00Z","timestamp":1542240000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["03ZZ0808A"],"award-info":[{"award-number":["03ZZ0808A"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2018,11,15]]},"abstract":"<jats:p>\n            In this study, an enzyme for imidazoline formation in pseudochelin biosynthesis was identified. Evidence for the involvement of this enzyme in the postulated reaction was obtained after\n            <jats:italic>in vitro<\/jats:italic>\n            reconstitution. Furthermore, the function of this enzyme was demonstrated\n            <jats:italic>in vivo<\/jats:italic>\n            by transferring the corresponding gene into the bacterium\n            <jats:named-content content-type=\"genus-species\">Myxococcus xanthus<\/jats:named-content>\n            , which thereby became a producer of pseudochelin A. In addition to clarifying the molecular basis of imidazoline formation in siderophore biosynthesis, we describe the heterologous expression of a gene in a myxobacterium without chromosomal integration. Due to its metabolic proficiency,\n            <jats:named-content content-type=\"genus-species\">M. xanthus<\/jats:named-content>\n            represents an interesting alternative to established host systems for the reconstitution and manipulation of biosynthetic pathways. Since the plasmid used in this study is easily adaptable for the expression of other enzymes as well, we expand the conventional expression strategy for myxobacteria, which is based on the integration of biosynthetic genes into the host genome.\n          <\/jats:p>","DOI":"10.1128\/aem.01789-18","type":"journal-article","created":{"date-parts":[[2018,9,11]],"date-time":"2018-09-11T13:21:37Z","timestamp":1536672097000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Engineering Pseudochelin Production in Myxococcus xanthus"],"prefix":"10.1128","volume":"84","author":[{"given":"Juliane","family":"Korp","sequence":"first","affiliation":[{"name":"Department of Biochemical and Chemical Engineering, Laboratory of Technical Biology, TU Dortmund University, Dortmund, Germany"},{"name":"Leibniz Institute for Natural Product Research and Infection Biology, Hans Kn\u00f6ll Institute, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lea","family":"Winand","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Chemical Engineering, Laboratory of Technical Biology, TU Dortmund University, Dortmund, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angela","family":"Sester","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Chemical Engineering, Laboratory of Technical Biology, TU Dortmund University, Dortmund, Germany"},{"name":"Leibniz Institute for Natural Product Research and Infection Biology, Hans Kn\u00f6ll Institute, Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0847-086X","authenticated-orcid":false,"given":"Markus","family":"Nett","sequence":"additional","affiliation":[{"name":"Department of Biochemical and Chemical Engineering, Laboratory of Technical Biology, TU Dortmund University, Dortmund, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-6445(03)00055-X"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.201100552"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6OB01400C"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.091208.073158"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2015.07.022"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2016.07.008"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1039\/c4np00011k"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.3762\/bjoc.12.58"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1039\/C6NP00106H"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.7164\/antibiotics.42.14"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200400206"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.02863-07"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1021\/np500909b"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1021\/ol501826a"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/ja.2006.93"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.4056\/sigs.2194987"},{"key":"e_1_3_3_18_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.2000.01740.x"},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.201167098"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja8025278"},{"key":"e_1_3_3_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.201500446"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tet.2017.03.051"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/s12864-015-1365-z"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0134(199705)28:1<72::AID-PROT7>3.0.CO;2-L"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi047326v"},{"key":"e_1_3_3_26_2","volume-title":"Biochemical pathways: an atlas of biochemistry and molecular biology","author":"Michal G","year":"1999","unstructured":"Michal G. 1999. 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