{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T14:37:34Z","timestamp":1792679854887,"version":"4.5.0"},"reference-count":60,"publisher":"American Society for Microbiology","issue":"20","license":[{"start":{"date-parts":[[2012,10,15]],"date-time":"2012-10-15T00:00:00Z","timestamp":1350259200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2012,10,15]]},"abstract":"ABSTRACT<\/jats:title>\n \n The kinetics of inorganic Hg [Hg(II)\n i<\/jats:sub>\n ] association, methylation, and methylmercury (MeHg) demethylation were examined for a group of\n Desulfovibrio<\/jats:named-content>\n species with and without MeHg production capability. We employed a detailed method for assessing MeHg production in cultures, including careful control of medium chemistry, cell density, and growth phase, plus mass balance of Hg(II)\n i<\/jats:sub>\n and MeHg during the assays. We tested the hypothesis that differences in Hg(II)\n i<\/jats:sub>\n sorption and\/or uptake rates drive observed differences in methylation rates among\n Desulfovibrio<\/jats:named-content>\n species. Hg(II)\n i<\/jats:sub>\n associated rapidly and with high affinity to both methylating and nonmethylating species. MeHg production by Hg-methylating strains was rapid, plateauing after \u223c3 h. All MeHg produced was rapidly exported. We also tested the idea that all\n Desulfovibrio<\/jats:named-content>\n species are capable of Hg(II)\n i<\/jats:sub>\n methylation but that rapid demethylation masks its production, but we found this was not the case. Therefore, the underlying reason why MeHg production capability is not universal in the\n Desulfovibrio<\/jats:named-content>\n is not differences in Hg affinity for cells nor differences in the ability of strains to degrade MeHg. However, Hg methylation rates varied substantially between Hg-methylating\n Desulfovibrio<\/jats:named-content>\n species even in these controlled experiments and after normalization to cell density. Thus, biological differences may drive cross-species differences in Hg methylation rates. As part of this study, we identified four new Hg methylators (\n Desulfovibrio aespoeensis<\/jats:named-content>\n ,\n D. alkalitolerans<\/jats:named-content>\n ,\n D. psychrotolerans<\/jats:named-content>\n , and\n D. sulfodismutans<\/jats:named-content>\n ) and four nonmethylating species (\n Desulfovibrio alcoholivorans<\/jats:named-content>\n ,\n D. tunisiensis<\/jats:named-content>\n ,\n D. carbinoliphilus<\/jats:named-content>\n , and\n D. piger<\/jats:named-content>\n ) in our ongoing effort to generate a library of strains for Hg methylation genomics.\n <\/jats:p>","DOI":"10.1128\/aem.01792-12","type":"journal-article","created":{"date-parts":[[2012,8,12]],"date-time":"2012-08-12T02:00:35Z","timestamp":1344736835000},"page":"7337-7346","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":100,"title":["Detailed Assessment of the Kinetics of Hg-Cell Association, Hg Methylation, and Methylmercury Degradation in Several Desulfovibrio Species"],"prefix":"10.1128","volume":"78","author":[{"given":"Andrew M.","family":"Graham","sequence":"first","affiliation":[{"name":"Smithsonian Environmental Research Center, Edgewater, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allyson L.","family":"Bullock","sequence":"additional","affiliation":[{"name":"Smithsonian Environmental Research Center, Edgewater, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew C.","family":"Maizel","sequence":"additional","affiliation":[{"name":"Smithsonian Environmental Research Center, Edgewater, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dwayne A.","family":"Elias","sequence":"additional","affiliation":[{"name":"Biosciences Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cynthia C.","family":"Gilmour","sequence":"additional","affiliation":[{"name":"Smithsonian Environmental Research Center, Edgewater, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chemosphere.2010.10.050"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/aoc.572"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1462-2920.2010.02260.x"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.67.1.51-58.2001"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1021\/es001415n"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1021\/es9808200"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/etc.5620181004"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.56.1.298-300.1990"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_3_3_11_2","doi-asserted-by":"publisher","DOI":"10.1029\/1999GB900033"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/etc.395"},{"key":"e_1_3_3_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2007.10.072"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.59.1.290-295.1993"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.4319\/lo.1999.44.7.1815"},{"key":"e_1_3_3_16_2","volume-title":"Standard methods for the examination of water and wastewater","author":"Clesceri LS","year":"2000","unstructured":"ClesceriLS GreenbergAE EatonAD (ed). 2000. 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