{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T19:52:43Z","timestamp":1789847563163,"version":"4.0.1"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T00:00:00Z","timestamp":1173916800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2007,3,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The atypical hemolytic\n            <jats:italic>Listeria innocua<\/jats:italic>\n            strains PRL\/NW 15B95 and J1-023 were previously shown to contain gene clusters analogous to the pathogenicity island (LIPI-1) present in the related foodborne gram-positive facultative intracellular pathogen\n            <jats:italic>Listeria monocytogenes<\/jats:italic>\n            , which causes listeriosis. LIPI-1 includes the hemolysin gene, thus explaining the hemolytic activity of the atypical\n            <jats:italic>L. innocua<\/jats:italic>\n            strains. No other\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            -specific virulence genes were found to be present. In order to investigate whether any other specific\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            genes could be identified, a global approach using a\n            <jats:italic>Listeria<\/jats:italic>\n            biodiversity DNA array was applied. According to the hybridization results, the isolates were defined as\n            <jats:italic>L. innocua<\/jats:italic>\n            strains containing LIPI-1. Surprisingly, evidence for the presence of the\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            -specific\n            <jats:italic>inlA<\/jats:italic>\n            gene, previously thought to be absent, was obtained. The\n            <jats:italic>inlA<\/jats:italic>\n            gene codes for the InlA protein which enables bacterial entry into some nonprofessional phagocytic cells. PCR and sequence analysis of this region revealed that the flanking genes of the\n            <jats:italic>inlA<\/jats:italic>\n            gene at the upstream, 5\u2032-end region were similar to genes found in\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            serotype 4b isolates, whereas the organization of the downstream, 3\u2032-end region was similar to that typical of\n            <jats:italic>L. innocua<\/jats:italic>\n            . Sequencing of the\n            <jats:italic>inlA<\/jats:italic>\n            region identified a small stretch reminiscent of the\n            <jats:italic>inlB<\/jats:italic>\n            gene of\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            . The presence of two clusters of\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            -specific genes makes it unlikely that PRL\/NW 15B95 and J1-023 are\n            <jats:italic>L. innocua<\/jats:italic>\n            strains altered by horizontal transfer. It is more likely that they are distinct relics of the evolution of\n            <jats:italic>L. innocua<\/jats:italic>\n            from an ancestral\n            <jats:italic>L. monocytogenes<\/jats:italic>\n            , as postulated by others.\n          <\/jats:p>","DOI":"10.1128\/aem.01796-06","type":"journal-article","created":{"date-parts":[[2007,1,13]],"date-time":"2007-01-13T01:35:27Z","timestamp":1168652127000},"page":"1928-1939","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["The Presence of the Internalin Gene in Natural Atypically Hemolytic\n            <i>Listeria innocua<\/i>\n            Strains Suggests Descent from\n            <i>L. monocytogenes<\/i>"],"prefix":"10.1128","volume":"73","author":[{"given":"Dmitriy V.","family":"Volokhov","sequence":"first","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20895"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandra","family":"Duperrier","sequence":"additional","affiliation":[{"name":"Laboratoire de Ge\u0301nomique des Microorganismes Pathoge\u0300nes, Institut Pasteur, 28 Rue du Dr. Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander A.","family":"Neverov","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20895"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"George","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Bethesda, Maryland 20895"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carmen","family":"Buchrieser","sequence":"additional","affiliation":[{"name":"Laboratoire de Ge\u0301nomique des Microorganismes Pathoge\u0300nes, Institut Pasteur, 28 Rue du Dr. Roux, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony D.","family":"Hitchins","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland 20740"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"221","volume":"132","year":"2000","unstructured":"Aiyar, A. 2000. The use of CLUSTAL W and CLUSTAL X for multiple sequence alignment. Methods Mol. Biol.132:221-241.","journal-title":"Methods Mol. Biol."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki063"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1605(89)90018-4"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(00)90365-4"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.7.2584-2592.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(02)02342-9"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3319-3325.2002"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1438-4221(00)80086-7"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.012363899"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.1998.00736.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.2.1072-1083.2004"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2002.03080.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90009-N"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1063447"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1605(00)00501-8"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg752"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1413"},{"key":"e_1_3_2_19_2","unstructured":"Hitchins A. 2003. Detection and enumeration of Listeria monocytogenes in foods. U.S. Food and Drug Administration Bacteriological Analytical Manual Online. U.S. Food and Drug Administration Washington DC. http:\/\/www.cfsan.fda.gov\/\u223cebam\/bam-10.html\n."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2006.09.011"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.7.4256-4266.2004"},{"key":"e_1_3_2_22_2","first-page":"97","year":"1999","unstructured":"Kuhn, M., and W. Goebel. 1999. Pathogenesis of Listeria monocytogenes, p. 97-130. In E. T. Ryser and E. H. Marth (ed.), Listeria, listeriosis, and food safety, 2nd ed. Marcel Dekker, Inc., New York, NY.","journal-title":"Listeria, listeriosis, and food safety"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1244"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.10.3896-3903.1995"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.70.4.2193-2203.2004"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkh562"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.187.16.5537-5551.2005"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1093\/genetics\/120.3.621"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.11.5308-5309.2003"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.10.3818"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302105"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.1.276-285.2004"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.146.6.1333"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.757-762.2003"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2004.09.005"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm1396"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.177.22.6469-6476.1995"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)00141-0"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01413-7"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.3.584-640.2001"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.72.4.2439-2448.2006"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.15.4994-5002.2004"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.182.3.837-841.2000"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.185.18.5573-5584.2003"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01796-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01796-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T16:32:23Z","timestamp":1645633943000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01796-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,3,15]]},"references-count":43,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2007,3,15]]}},"alternative-id":["10.1128\/AEM.01796-06"],"URL":"https:\/\/doi.org\/10.1128\/aem.01796-06","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,3,15]]},"assertion":[{"value":"2006-07-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2006-12-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-03-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}