{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:34:20Z","timestamp":1791192860087,"version":"4.1.0"},"reference-count":62,"publisher":"American Society for Microbiology","issue":"18","license":[{"start":{"date-parts":[[2013,9,15]],"date-time":"2013-09-15T00:00:00Z","timestamp":1379203200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2013,9,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n            is a food-borne bacterial pathogen and the causative agent of human and animal listeriosis. Among the three major genetic lineages of\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            (i.e., LI, LII, and LIII), LI and LII are predominantly associated with food-borne listeriosis outbreaks, whereas LIII is rarely implicated in human infections. In a previous study, we identified a Crp\/Fnr family transcription factor gene,\n            <jats:italic>lmo0753<\/jats:italic>\n            , that was highly specific to outbreak-associated LI and LII but absent from LIII. Lmo0753 shares two conserved functional domains, including a DNA binding domain, with the well-characterized master virulence regulator PrfA in\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            . In this study, we constructed\n            <jats:italic>lmo0753<\/jats:italic>\n            deletion and complementation mutants in two fully sequenced\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            LII strains, 10403S and EGDe, and compared the flagellar motility, phospholipase C production, hemolysis, and intracellular growth of the mutants and their respective wild types. Our results suggested that\n            <jats:italic>lmo0753<\/jats:italic>\n            plays a role in hemolytic activity in both EGDe and 10403S. More interestingly, we found that deletion of\n            <jats:italic>lmo0753<\/jats:italic>\n            led to the loss of\n            <jats:sc>l<\/jats:sc>\n            -rhamnose utilization in EGDe, but not in 10403S. RNA-seq analysis of EGDe \u0394\n            <jats:italic>0753<\/jats:italic>\n            incubated in phenol red medium containing\n            <jats:sc>l<\/jats:sc>\n            -rhamnose as the sole carbon source revealed that 126 (4.5%) and 546 (19.5%) out of 2,798 genes in the EGDe genome were up- and downregulated more than 2-fold, respectively, compared to the wild-type strain. Genes related to biotin biosynthesis, general stress response, and rhamnose metabolism were shown to be differentially regulated. Findings from this study collectively suggested varied functional roles of\n            <jats:italic>lmo0753<\/jats:italic>\n            in different LII\n            <jats:named-content content-type=\"genus-species\">L. monocytogenes<\/jats:named-content>\n            strain backgrounds associated with human listeriosis outbreaks.\n          <\/jats:p>","DOI":"10.1128\/aem.01812-13","type":"journal-article","created":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T01:55:33Z","timestamp":1373334933000},"page":"5584-5592","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["<i>prfA<\/i>\n            -Like Transcription Factor Gene\n            <i>lmo0753<\/i>\n            Contributes to\n            <scp>l<\/scp>\n            -Rhamnose Utilization in Listeria monocytogenes Strains Associated with Human Food-Borne Infections"],"prefix":"10.1128","volume":"79","author":[{"given":"Joelle K.","family":"Salazar","sequence":"first","affiliation":[{"name":"Institute for Food Safety and Health, Illinois Institute of Technology, Bedford Park, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhuchun","family":"Wu","sequence":"additional","affiliation":[{"name":"Institute for Food Safety and Health, Illinois Institute of Technology, Bedford Park, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. David","family":"McMullen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qin","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Life Science, Huazhong Normal University, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy E.","family":"Freitag","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, University of Illinois College of Medicine, Chicago, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Lou","family":"Tortorello","sequence":"additional","affiliation":[{"name":"U.S. Food and Drug Administration, Bedford Park, Illinois, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shencai","family":"Hu","sequence":"additional","affiliation":[{"name":"School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute for Food Safety and Health, Illinois Institute of Technology, Bedford Park, Illinois, USA"},{"name":"School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.4315\/0362-028X-65.11.1811"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/mr.55.3.476-511.1991"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2171"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2007.05.011"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0505.990502"},{"key":"e_1_3_3_7_2","first-page":"749","article-title":"Vital signs: incidence and trends of infection with pathogens transmitted commonly through food\u2014foodborne diseases active surveillance network, 10 U.S. sites, 1996\u20132010","volume":"60","author":"CDC","year":"2011","unstructured":"CDC. 2011. 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