{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T17:39:55Z","timestamp":1793295595830,"version":"4.5.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2011,1,15]],"date-time":"2011-01-15T00:00:00Z","timestamp":1295049600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["Appl Environ Microbiol"],"published-print":{"date-parts":[[2011,1,15]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Melioidosis is a major cause of morbidity and mortality in Southeast Asia, where the causative organism (\n            <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n            ) is present in the soil. In the Lao People's Democratic Republic (Laos),\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            is a significant cause of sepsis around the capital, Vientiane, and has been isolated in soil near the city, adjacent to the Mekong River. We explored whether\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            occurs in Lao soil distant from the Mekong River, drawing three axes across northwest, northeast, and southern Laos to create nine sampling areas in six provinces. Within each sampling area, a random rice field site containing a grid of 100 sampling points each 5 m apart was selected. Soil was obtained from a depth of 30 cm and cultured for\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            . Four of nine sites (44%) were positive for\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            , including all three sites in Saravane Province, southern Laos. The highest isolation frequency was in east Saravane, where 94% of soil samples were\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            positive with a geometric mean concentration of 464 CFU\/g soil (95% confidence interval, 372 to 579 CFU\/g soil; range, 25 to 10,850 CFU\/g soil). At one site in northwest Laos (Luangnamtha), only one sample (1%) was positive for\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            , at a concentration of 80 CFU\/g soil. Therefore,\n            <jats:italic>B. pseudomallei<\/jats:italic>\n            occurs in Lao soils beyond the immediate vicinity of the Mekong River, alerting physicians to the likelihood of melioidosis in these areas. Further studies are needed to investigate potential climatic, soil, and biological determinants of this heterogeneity.\n          <\/jats:p>","DOI":"10.1128\/aem.01822-10","type":"journal-article","created":{"date-parts":[[2010,11,13]],"date-time":"2010-11-13T05:59:41Z","timestamp":1289627981000},"page":"532-536","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Randomized Soil Survey of the Distribution of\n            <i>Burkholderia pseudomallei<\/i>\n            in Rice Fields in Laos"],"prefix":"10.1128","volume":"77","author":[{"given":"Sayaphet","family":"Rattanavong","sequence":"first","affiliation":[{"name":"Institut de la Francophonie pour la Me\u0301decine Tropicale, BP 9519, Vientiane, Lao People's Democratic Republic"},{"name":"Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vanaporn","family":"Wuthiekanun","sequence":"additional","affiliation":[{"name":"Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sayan","family":"Langla","sequence":"additional","affiliation":[{"name":"Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Premjit","family":"Amornchai","sequence":"additional","affiliation":[{"name":"Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy","family":"Sirisouk","sequence":"additional","affiliation":[{"name":"Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rattanaphone","family":"Phetsouvanh","sequence":"additional","affiliation":[{"name":"Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catrin E.","family":"Moore","sequence":"additional","affiliation":[{"name":"Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sharon J.","family":"Peacock","sequence":"additional","affiliation":[{"name":"Mahidol Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand"},{"name":"Department of Medicine, University of Cambridge, Addenbrooke's Hospital, Cambridge, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Buisson","sequence":"additional","affiliation":[{"name":"Institut de la Francophonie pour la Me\u0301decine Tropicale, BP 9519, Vientiane, Lao People's Democratic Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul N.","family":"Newton","sequence":"additional","affiliation":[{"name":"Wellcome Trust-Mahosot Hospital-Oxford Tropical Medicine Research Collaboration, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/159.5.890"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01720-09"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.18.2.383-416.2005"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/318116"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2007.11.005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.4.1.52"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-7061(97)00031-1"},{"key":"e_1_3_2_9_2","unstructured":"World soil resources report no. 60 1988"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00013542-200101000-00002"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01038-07"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000364"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000694"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268809990264"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2003.10.003"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2007.06.004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1086\/313479"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/318713"},{"key":"e_1_3_2_19_2","first-page":"416","volume":"89","year":"1993","unstructured":"Phung, L. V., H. T. Quynh, E. Yabuuchi, and D. A. B. Dance. 1993. Pilot study of exposure to Pseudomonas pseudomallei in northern Vietnam. Trans. R. Soc. Trop. Med. Hyg. 89:416.","journal-title":"Trans. R. Soc. Trop. Med. Hyg."},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1080\/00103629509369453"},{"key":"e_1_3_2_21_2","unstructured":"Atlas of Laos. 2000"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90078-0"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/520223"},{"key":"e_1_3_2_24_2","unstructured":"United Nations Development Programme. 2008. Hazardous ground-cluster munitions and UXO in the Lao PDR. United Nations Development Programme Vientiane Laos. http:\/\/www.undplao.org\/newsroom\/publication\/Hazardous_Ground_FINAL.pdf."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.458"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13374-0"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(95)90651-7"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2002.66.759"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.2.923-924.2005"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1402.070811"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000496"}],"container-title":["Applied and Environmental Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01822-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/AEM.01822-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T17:23:45Z","timestamp":1645637025000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/AEM.01822-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,1,15]]},"references-count":30,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,1,15]]}},"alternative-id":["10.1128\/AEM.01822-10"],"URL":"https:\/\/doi.org\/10.1128\/aem.01822-10","relation":{},"ISSN":["0099-2240","1098-5336"],"issn-type":[{"value":"0099-2240","type":"print"},{"value":"1098-5336","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,1,15]]},"assertion":[{"value":"2010-07-27","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-10-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-01-08","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}